FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990764
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGCTCTCAGACTGCTCATTTGCAGATACA29100.2937127307814979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24850.25081654157801453No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24360.24587086329337765No Hit
GTTTATTACTGTGCGAAGGATGACGTAGCAGTGACTGGTATGGGTCACCT23220.23436459136585505No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22790.23002450634056143No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21420.21619679358555619No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21210.21407721717785466No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19310.19490009729865035No Hit
GTGTGAGGTGCAGCTGTTGGAGTCGGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC19120.1929823853107299No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19060.1923767920513866No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18000.1816779778029884No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17170.17330060438207284No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16620.16774933283809262No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16500.16653814631940606No Hit
GGCCAGGCCAGTCCCCTGTGTTGGTCATCTATCAAGATACTAAGCGGCCC16260.16411577328203286No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16230.1638129766523612No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16220.16371204444247067No Hit
GGGCTAGAGTGGGTCTCAGCTGTCAGTGGCAGTGGTAGTAGGACATACTA16190.163409247812799No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16030.16179433245455022No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA15570.15715145079958495No Hit
CTGCTACGTCATCCTTCGCACAGTAATAAACGGCCGTGTCCTCGGCTCTC15370.155132806601774No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15370.155132806601774No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15250.1539216200830874No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15200.15341695903363467No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14160.14292000920501755No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13630.13757060208081845No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13570.13696500882147516No Hit
ACGTAGCAGTGACTGGTATGGGTCACCTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCACCATCCC13470.13595568672256964No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13300.1342398391544303No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGTTGGG13250.13373517810497756No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13230.1335333136851965No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGGTGCTGCTGTCCCACGCCTGACAGTAATAGT13220.13343238147530592No Hit
CCCCTGTGTTGGTCATCTATCAAGATACTAAGCGGCCCTCAGGGATCCCT13140.13262492379618152No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13110.1323221271665099No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12940.13060627959837057No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAGGTGACCCATAC12830.12949602528957452No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12810.12929416086979342No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12730.12848670319066902No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12680.12798204214121625No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12580.12697272004231078No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12580.12697272004231078No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12580.12697272004231078No Hit
CTCTAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATGGCATAGCTGC12470.12586246573351473No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG12380.12495407584449979No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12330.12444941479504705No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12160.12273356722690772No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12050.12162331291811167No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11850.1196046687203007No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACCC11670.11778788894227081No Hit
GTCTGAGAGCCGAGGACACGGCCGTTTATTACTGTGCGAAGGATGACGTA11590.11698043126314642No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG11510.11617297358402204No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACCCATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT11510.11617297358402204No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11480.11587017695435038No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11430.11536551590489764No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11260.11364966833675831No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11250.11354873612686775No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGGTGCTGCTGTCCCACGCCTGA11240.1134478039169772No Hit
GGGTTCCCTGGCCCCAGAAGTCAAGGTGACCCATACCAGTCACTGCTACGTCATCCTTCGCACAGTAATAAACGG10910.11011704099058908No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGGCCA10900.11001610878069853No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10710.10809839679277809No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATGGGTGCTGC10660.10759373574332534No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACCCATTATGTCTTCGGAACTG10600.10698814248398206No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC10590.10688721027409152No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10510.10607975259496712No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10330.10426297281693722No Hit
GTGTTGGTCATCTATCAAGATACTAAGCGGCCCTCAGGGATCCCTGAGCG10280.10375831176748448No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT10260.10355644734770339No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGGTTCCCTGG10220.1031527185081412No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10090.10184059977956406No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG10080.1017396675696735No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10020.10113407431033021No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10000.10093220989054912No Hit
GGCCAGTCCCCTGTGTTGGTCATCTATCAAGATACTAAGCGGCCCTCAGG9970.10062941326087747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGGA100.009616694130.941870
TAGTGCC200.007395117551.68544837
TTTCGAC200.007680744451.192413
GTGGTAT2700.040.460581
TCTGGAA12400.039.07134670
CGTGTGT400.002737112334.1299976
AATGGTA3400.032.7354570
GTATCAA21200.032.689331
TATCAAC24750.027.7218912
CACTGCG500.00797704527.42581630
ATTGTCT11600.026.86822164
TTGTCTC11850.026.69562565
ATACGTA907.511031E-626.54421211
AATACGT907.511031E-626.54421210
CATATTC12050.026.16346452
ATCAACG26400.025.9840263
AATTGTC12100.025.69805563
ATTCTTG12200.025.6730255
TCAACGC26650.025.6122134
TAGTCAC1359.46784E-925.54450638
Produced by FastQC (version 0.11.5)