FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432220
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC89040.6216921981259863No Hit
GCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACCACGGAAATGGTGTGATCATAAGA78810.5502646241499212No Hit
GCCCCATTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGCCAGCGGTGACAGCATTGGCAG70860.49475639217438666No Hit
CTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCT52090.36370110737177247No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50030.3493178422309422No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48110.3359120805462848No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTC47570.3321417100724749No Hit
CTGCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAA40180.28054349192163214No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39890.27851866333384534No Hit
GACTACTACTGTCAGTCTTATGATCACACCATTTCCGTGGTGGTCTTCGG36910.2577118040524501No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34830.2431888955607379No Hit
GTAGTAGTCAGCCTCGTCCTCAGACTTCAGCCCAGAGATGGTGAGGGAGG34260.23920906006060522No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33720.2354386895867953No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32950.23006242057784418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32520.22706008853388446No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31190.21777380570024157No Hit
CTACTACTGTCAGTCTTATGATCACACCATTTCCGTGGTGGTCTTCGGCG30800.21505076035804555No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA30550.21330521847202247No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30510.21302593177025875No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29870.2085573445420396No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGCCAGCGGTGACAGCATTGGCAGCCACTATATG29060.2029017888313248No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28710.20045803019089248No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28250.19724623312061No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28110.19626872966443704No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28070.19598944296267332No Hit
GTTTGGTCCCTCCGCCGAAGACCACCACGGAAATGGTGTGATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCT27620.19284746756783178No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27500.19200960746254067No Hit
CCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCTC26910.18789012861152615No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGACTTCAGCCCAGAGATGGTGAGGGA26810.18719191185711692No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26380.1841895798131572No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT25340.17692812556730111No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT24960.174274901900546No Hit
GTTCTCTGGCTCCATCGACACCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGGCTGAAGTCTGAGGACGAGG24860.17357668514613678No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24830.17336722011981398No Hit
GAGTTGGAGGAGGTGTCGATGGAGCCAGAGAACCGAGCAGGGACCCCAGA24760.17287846839172755No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24570.17155185655834998No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24100.16827023781262654No Hit
GTCTTATGATCACACCATTTCCGTGGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGG23910.166943625979249No Hit
CCCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCT23670.16526790576866682No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGA23560.16449986733881666No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACCA23540.16436022398793482No Hit
CACCATTTCCGTGGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGT23380.16324307718088002No Hit
TCTCTGGGCTGAAGTCTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT23150.1616371786457388No Hit
GAAATGGTGTGATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGA22850.15954252838251107No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22820.1593330633561883No Hit
CACCATCTCTGGGCTGAAGTCTGAGGACGAGGCTGACTACTACTGTCAGT22070.154096437698119No Hit
GGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21760.15193196575945037No Hit
CTCCTGCACCGCCAGCGGTGACAGCATTGGCAGCCACTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC21720.15165267905768665No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21160.14774266523299495No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTC21110.1473935568557903No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCC20960.14634623172417646No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20880.14578765832064905No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20760.14494979821535797No Hit
CCCCAGAGGGTAGTCGAGAATTTTCATAGATAACCATAAGGGGGGCACTG20660.14425158146094874No Hit
CCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGG20610.14390247308374413No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20410.14250603957492564No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20400.1424362178994847No Hit
GCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATTCTGTGTCG20090.14027174596081607No Hit
CCATAAGGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCATATAGTGG19820.13838656072391115No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGGCTGAAGTCTGAGGACGAG19450.13580315873259696No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATT19080.13321975674128278No Hit
GGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTC19060.1330801133904009No Hit
GACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACCACGGAAATG18990.13259136166231444No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18850.13161385820614152No Hit
GAGATGGTGAGGGAGGCAGAGTTGGAGGAGGTGTCGATGGAGCCAGAGAACCGAGCAGGGACCCCAGAGGGTAGT18780.13112510647805506No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18570.12965885129379565No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18110.12644705422351316No Hit
ACCCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCTCCTCCA18030.12588848081998574No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17620.12302579212690788No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGACTTCAGCCCAGA17580.12274650542514418No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17440.12176900196897125No Hit
GAATGGGGCTGAGTCAGCAAAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC17310.12086132018823924No Hit
GCATATAGTGGCTGCCAATGCTGTCACCGCTGGCGGTGCAGGAGATGTTTACCGTCTTCCCCGGAGACTCCGACA17310.12086132018823924No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17300.12079149851279831No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17130.11960453003030261No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCT17120.11953470835486169No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17010.11876666992501153No Hit
ACTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCT17000.1186968482495706No Hit
CTGCATATAGTGGCTGCCAATGCTGTCACCGCTGGCGGTGCAGGAGATGTTTACCGTCTTCCCCGGAGACTCCGA16640.11618326793369733No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGACT16340.11408861767046963No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16320.11394897431958777No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT16150.11276200583709207No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15840.11059753389842343No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15370.10731591515270002No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15370.10731591515270002No Hit
GTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTC15360.10724609347725908No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15120.10557037326667691No Hit
ACCATAAGGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCATATAGTG15080.1052910865649132No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG14950.1043834047841812No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTCCCTG14800.10333607965256734No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14710.10270768457359904No Hit
CCACTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACC14690.10256804122271718No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14600.10193964614374885No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14590.10186982446830793No Hit
GTCTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATCACACCATTTCCGTGGTGGTCTTCGGCGGAGGGAC14330.10005446090684393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)