FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432220
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC84110.5872701121336108No Hit
GCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACCACGGAAATGGTGTGATCATAAGA72670.5073941154291939No Hit
GCCCCATTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGCCAGCGGTGACAGCATTGGCAG65400.4566337573836422No Hit
CTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCT50690.3539260728100431No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50270.35099356244152435No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48740.340310846099063No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43130.30114088617670465No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTC42570.29723087235201295No Hit
CTGCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAA38510.26888327212299784No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36710.25631537054363157No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36120.2521958916926171No Hit
GACTACTACTGTCAGTCTTATGATCACACCATTTCCGTGGTGGTCTTCGG35600.24856516456968902No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34450.24053567189398278No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG33560.23432154277974057No Hit
GTAGTAGTCAGCCTCGTCCTCAGACTTCAGCCCAGAGATGGTGAGGGAGG33320.2326458225691584No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32020.22356900476183825No Hit
CTACTACTGTCAGTCTTATGATCACACCATTTCCGTGGTGGTCTTCGGCG31150.21749451899847788No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30620.21379397020010893No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30460.21267682339305413No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTCCTGCACCGCCAGCGGTGACAGCATTGGCAGCCACTATATG29670.20716091103322115No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29350.2049266174191116No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA29020.20262250212956112No Hit
CCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCTC27630.19291728924327267No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27430.1915208557344542No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27100.18921674044490372No Hit
GTTTGGTCCCTCCGCCGAAGACCACCACGGAAATGGTGTGATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCT26810.18719191185711692No Hit
GTTCTCTGGCTCCATCGACACCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGGCTGAAGTCTGAGGACGAGG26790.18705226850623508No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26450.18467833154124366No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25590.17867366745332422No Hit
GTCTTATGATCACACCATTTCCGTGGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGG24940.17413525854966416No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGACTTCAGCCCAGAGATGGTGAGGGA24780.17301811174260937No Hit
GAGTTGGAGGAGGTGTCGATGGAGCCAGAGAACCGAGCAGGGACCCCAGA24710.1725293600145229No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24390.17029506640041336No Hit
CCCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCT23530.1642904023124939No Hit
TCTCTGGGCTGAAGTCTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT23480.16394129393528928No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23430.16359218555808464No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACCA22840.15947270670707014No Hit
CACCATTTCCGTGGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGT22590.15772716482104704No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22380.15626090963678765No Hit
CCCCAGAGGGTAGTCGAGAATTTTCATAGATAACCATAAGGGGGGCACTG22180.15486447612796916No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21850.15256036083841867No Hit
CACCATCTCTGGGCTGAAGTCTGAGGACGAGGCTGACTACTACTGTCAGT21820.1523508958120959No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21670.15130357068048203No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT21590.15074499727695467No Hit
GAAATGGTGTGATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGA21580.15067517560151375No Hit
CCATAAGGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCATATAGTGG20850.1455781932943263No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20840.14550837161888536No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20720.14467051151359428No Hit
CTCCTGCACCGCCAGCGGTGACAGCATTGGCAGCCACTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC20720.14467051151359428No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20710.14460068983815336No Hit
CCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGG20660.14425158146094874No Hit
GGACCAAACTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG20480.14299479130301213No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20470.14292496962757117No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20270.1415285361187527No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20180.1409001410397844No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20080.14020192428537515No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGTTTGGTC20070.14013210260993425No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19870.13873566910111576No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGGCTGAAGTCTGAGGACGAG19760.1379676306712656No Hit
GGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAACATCTC19380.13531440700451047No Hit
GCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATTCTGTGTCG19180.133917973495692No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGA19170.13384815182025106No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19080.13321975674128278No Hit
ACCCTCTGGGGTCCCTGCTCGGTTCTCTGGCTCCATCGACACCTCCTCCA18930.1321724316096689No Hit
CTGCATATAGTGGCTGCCAATGCTGTCACCGCTGGCGGTGCAGGAGATGTTTACCGTCTTCCCCGGAGACTCCGA18370.12826241778497716No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT18190.12700562762704054No Hit
ACCATAAGGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCATATAGTG17370.12128025024088479No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17340.12107078521456201No Hit
GCATATAGTGGCTGCCAATGCTGTCACCGCTGGCGGTGCAGGAGATGTTTACCGTCTTCCCCGGAGACTCCGACA17210.12016310343382999No Hit
ACTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCT17090.1193252433285389No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGACTTCAGCCCAGA16970.11848738322324781No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16850.11764952311795673No Hit
GAGATGGTGAGGGAGGCAGAGTTGGAGGAGGTGTCGATGGAGCCAGAGAACCGAGCAGGGACCCCAGAGGGTAGT16550.11555487285472901No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16490.11513594280208347No Hit
GTCTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATCACACCATTTCCGTGGTGGTCTTCGGCGGAGGGAC16230.11332057924061947No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACCCTCTGGGGTCCCTG16110.11248271913532838No Hit
GACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACCACGGAAATG16110.11248271913532838No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCC15870.11080699892474619No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15850.11066735557386435No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15240.106408233371968No Hit
GAATGGGGCTGAGTCAGCAAAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC15160.1058496599684406No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTTTGCTGACTCAGCCCCATT15040.10501179986314951No Hit
CCACTATATGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCCTTATGGTTATCTATGAAAATTCTCGACTACC14980.10459286981050397No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14930.10424376143329936No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14840.10361536635433102No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14780.10319643630168548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.043.970031
GTGGTAT3500.042.8870281
TGGTATC3800.039.488792
GTATCAA31450.035.904481
TATCAAC36600.030.6562862
ATTAGAC450.004899177730.30513
TCAACGC37400.030.169214
ATCAACG37300.030.0715123
CAACGCA38600.029.319625
AACGCAG39000.028.932516
ATGGCAA3600.028.8656370
ACTGGTG42650.028.58814270
ACGCAGA41250.027.3543767
CGCAGAG41550.027.1568688
AGAGTAC42250.026.62531711
GCCGAGT808.0270926E-526.5498958
CAGAGTA43100.026.02113210
GTGTTAG1058.477109E-725.9921361
ACGGGTT3750.025.86360570
ACGTACC2250.025.75393913
Produced by FastQC (version 0.11.5)