FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118627
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39870.3564190744546663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39260.3509659609503436No Hit
GGGTAACAGTTTCCCGTACACCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCAT33290.2975969648506607No Hit
CTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33280.2975075695473111No Hit
CCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCA30070.26881167717210475No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28590.25558117227637095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26080.23314295113563321No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24170.21606844819586868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23590.21088352060159463No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22760.20346371042358177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22600.20203338556998895No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22100.1975636204025113No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20230.18084669867614495No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20210.18066790806944585No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19510.17441023683497717No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19380.17324809789143297No Hit
GGCCAAAGGTGTACGGGAAACTGTTACCCTGTTGACAATAGTAAGTTGCA18200.16269945209618578No Hit
GGATACTAGCAGATGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGGTGCATC18000.16091154602919472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17950.16046456951244695No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17950.16046456951244695No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17820.15930243056890275No Hit
GATCTACGGTGCATCCAGTTTATTTAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA17760.15876605874880545No Hit
GGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCATCTGTCTTCATC17690.1581402916253586No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17310.1547432700980756No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16860.15072048144734573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16670.1490219706837042No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16220.14499918203297435No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACAGTTTC15620.13963546383200118No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15260.1364172329114173No Hit
GTGCAGCCACAGTTCGAATGATCTCCAGCCTAGTCCCCTGGCCAAAGGTG14710.1315004912271919No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT14600.13051714289034683No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14240.12729891196976295No Hit
GCCTAGTCCCCTGGCCAAAGGTGTACGGGAAACTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG14140.1264049589362674No Hit
AGTCAGGATACTAGCAGATGGTTAGCCTGGTATCAGCAGAAACCAGGGAA14010.12524281999272321No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13890.12417007635252858No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13800.12336551862238263No Hit
GTGGGAGACAGAGTCACCATTACTTGTCGGGCGAGTCAGGATACTAGCAGATGGTTAGCCTGGTATCAGCAGAAA13710.12256096089223664No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGAATGATCTCCAGCCTAGTCCCCTGGCCAAAGGTGTACGGGAAA13670.12220337967883843No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13410.11987910179175007No Hit
CCATTACTTGTCGGGCGAGTCAGGATACTAGCAGATGGTTAGCCTGGTAT13310.11898514875825453No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13060.11675026617451573No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12790.1143365929840778No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12720.11371082586063093No Hit
ATTTAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATT12630.11290626813048497No Hit
TGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGAT12530.11201231509698943No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12480.11156533858024167No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA12480.11156533858024167No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12250.10950924660320197No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC12220.10924106069315331No Hit
CCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC12060.10781073583956047No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGAATCACTCTCAGTAACGCCTGG12030.10754254992951182No Hit
GGGTATAACATTTTGACTGCCTGGGGCCACGGAACCCCGGTCACCGTCTC11980.10709557341276403No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG11950.10682738750271538No Hit
ATTGTCAACAGGGTAACAGTTTCCCGTACACCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTG11870.10611222507591896No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11740.10495008613237479No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11620.10387734249218016No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11550.10325157536873328No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11480.10262580824528642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11350.10146366930174222No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTGGGAGACAGAGTCACCATTACTT11330.10128487869504313No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11310.10110608808834402No Hit
GTATAACATTTTGACTGCCTGGGGCCACGGAACCCCGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT11290.1009272974816449No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11210.1002121350548485No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11210.1002121350548485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)