FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118627
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37410.3344278298306763No Hit
CTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33450.2990272897042535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33410.29866970849085533No Hit
GGGTAACAGTTTCCCGTACACCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCAT33260.297328778940612No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29770.2661298180716182No Hit
CCTTTGGCCAGGGGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCA27890.24932350104190226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25770.23037169673179708No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24930.2228624912504347No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23400.20918500983795313No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21020.18790892764075962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20070.17941637382255213No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19130.17101321530769417No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18930.16922530924070311No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18720.1673480078703625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18690.16707982196031385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18600.1662752642301679No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18380.1643085675564777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18190.16261005679283622No Hit
GGCCAAAGGTGTACGGGAAACTGTTACCCTGTTGACAATAGTAAGTTGCA17530.15670996677176574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17430.15581601373827023No Hit
GATCTACGGTGCATCCAGTTTATTTAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA17110.15295536403108453No Hit
GTGGGAGACAGAGTCACCATTACTTGTCGGGCGAGTCAGGATACTAGCAGATGGTTAGCCTGGTATCAGCAGAAA16950.1515250391774917No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16880.15089927205404483No Hit
GGACTAGGCTGGAGATCATTCGAACTGTGGCTGCACCATCTGTCTTCATC16840.1505416908406466No Hit
GGATACTAGCAGATGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGGTGCATC16740.1496477378071511No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16080.1437476477860806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16080.1437476477860806No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16040.1433900665726824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15700.14035062625879763No Hit
GTGCAGCCACAGTTCGAATGATCTCCAGCCTAGTCCCCTGGCCAAAGGTG15630.13972485913535074No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15300.1367748141248155No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACAGTTTC15130.1352550939678731No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14330.1281034696999089No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14080.1258685871161701No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13800.12336551862238263No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13540.12104124073529425No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGAATGATCTCCAGCCTAGTCCCCTGGCCAAAGGTGTACGGGAAA13120.11728663799461304No Hit
GCCTAGTCCCCTGGCCAAAGGTGTACGGGAAACTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG13000.11621389435441841No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12830.11469417419747602No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12820.11460477889412646No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA12700.11353203525393182No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12680.11335324464723272No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12660.11317445404053363No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA12590.11254868691708675No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12210.10915166538980375No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12070.10790013114291001No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGAATCACTCTCAGTAACGCCTGG12050.10772134053621092No Hit
CCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC11980.10709557341276403No Hit
AGTCAGGATACTAGCAGATGGTTAGCCTGGTATCAGCAGAAACCAGGGAA11920.10655920159266673No Hit
CCATTACTTGTCGGGCGAGTCAGGATACTAGCAGATGGTTAGCCTGGTAT11480.10262580824528642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11460.10244701763858731No Hit
GGGTATAACATTTTGACTGCCTGGGGCCACGGAACCCCGGTCACCGTCTC11410.10200004112183954No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11370.10164245990844133No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11330.10128487869504313No Hit
GTATAACATTTTGACTGCCTGGGGCCACGGAACCCCGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT11230.10039092566154759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)