FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077240
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGA52820.4903271323010657No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35650.33093832386469124No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35230.32703947124132043No Hit
CAATAATACACTGCGAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32910.3055029519884148No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29030.2694849801344176No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA27370.25407522928966614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26720.24804129070587796No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24160.22427685566818906No Hit
GTGTATTATTGTCAGCAGTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23990.22269874865396755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23790.22084215216664813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21730.2017192083472578No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21550.2000482715086703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20970.1946641416954439No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20790.19299320485685642No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20160.18714492592180015No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT18570.17238498384761056No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18110.16811481192677583No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17990.16700085403438417No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17910.16625821543945637No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCTTCAGGGAGA17680.164123129479039No Hit
GTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17670.16403029965467306No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17520.16263785228918345No Hit
ATAATACACTGCGAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17370.16124540492369388No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGAGACCAGGGGACCTGGT16890.15678957335412722No Hit
GAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC16040.14889903828301956No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16030.1488062084586536No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT15660.14537150495711262No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCT15600.14481452601091677No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCCGGGTGAG14680.13627418216924733No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14640.13590286287178344No Hit
GACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTCGCAGTGTATTATTGTCAGCAGTATGGCAGCTCA14050.13042590323419107No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGGTCCTTAGGCTGGTT13830.12838364709813968No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC13800.12810515762504177No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13760.12773383832757787No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT13640.1266198804351862No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13570.12597007166462443No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGATCCTGTCTGGGAGACCA13400.12439196465040286No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13370.12411347517730498No Hit
GTCAGAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG13190.12244253833871746No Hit
GTCTCCGGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT13000.12067877167576399No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12950.12021462255393411No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12670.11761538747168691No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12410.11520181203817162No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12180.11306672607775427No Hit
GTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA12150.11278823660465635No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11870.11018900152240912No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11820.10972485240057926No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11640.10805391556199175No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT11580.10749693661579593No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC11340.10526902083101258No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11150.10350525416805911No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11140.10341242434369315No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11060.10266978574876535No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11000.10211280680256954No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10980.10192714715383758No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10810.10034904013961606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGA100.008031492139.0760370
GGTATCA14400.042.0099531
GTGGTAT3800.040.0212441
CGTATGC2650.039.1108441
AACTGTG21850.038.19021670
GTATCAA37200.037.9747541
TCGTATG2800.036.95948840
TACTGAT7150.036.95726870
CGAGACG2950.035.53228423
ATCTCGT3000.035.4154737
TGGTATC4300.035.3676072
ACGAGAC3150.034.34012622
TATCAAC41750.033.9980852
ATCAACG42150.033.7556273
TCAACGC42500.033.477644
CAACGCA43550.032.7480855
ATGCCGT3200.032.56639544
AACGCAG43950.032.3007436
CATCTCG3350.031.66912536
TATGCCG3300.031.52688243
Produced by FastQC (version 0.11.5)