FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077240
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGA49850.46275667446437185No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34810.32314061861794957No Hit
CAATAATACACTGCGAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31120.2888864134269058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29080.2699491292562474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28890.26818536259329395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27130.2518473135048829No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26430.2453492257992648No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA24660.22891834688648768No Hit
GTGTATTATTGTCAGCAGTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA22830.21193048902751477No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22300.20701050833611823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20910.19410716274924808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20430.18965133117968141No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19860.18436003119082098No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19010.17646949611971335No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18270.16960008911663138No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18110.16811481192677583No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18020.16727934350748208No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16910.15697523300285918No Hit
GTCCCAGACCCACTGCCACTGATCCTGTCTGGGAGACCAGGGGACCTGGT16680.1548401470424418No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16670.15474731721807583No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAACTCAGCTTCAGGGAGA16630.15437599792061193No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT16440.15261223125765846No Hit
GAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC16170.1501058259997772No Hit
ATAATACACTGCGAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16050.14899186810738554No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15870.14732093126879803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15410.14305075934796332No Hit
GTATGGCAGCTCACCCGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15190.1410085032119119No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14900.13831643830529872No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14860.13794511900783482No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCT14100.13089005235602094No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCCGGGTGAG14050.13042590323419107No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13790.1280123278006758No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGATCCTGTCTGGGAGACCA13540.12569158219152649No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGGTCCTTAGGCTGGTT13530.12559875236716053No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC13370.12411347517730498No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT13150.12207121904125356No Hit
GACTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTCGCAGTGTATTATTGTCAGCAGTATGGCAGCTCA13130.12188555939252163No Hit
GTCAGAGTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG12290.11408785414577995No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12230.11353087519958413No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11730.10888938398128552No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11690.10851806468382162No Hit
GTCTCCGGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT11580.10749693661579593No Hit
GTGTTACCAGGTCCTTAGGCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA11460.10638297872340426No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11340.10526902083101258No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11270.10461921206045077No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11250.10443355241171884No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11170.10369091381679106No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC11050.10257695592439939No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11030.10239129627566744No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10840.10062752961271398No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10830.10053469978834799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT651.6752892E-977.39059470
GTGGTAT4500.045.7464261
CTAACGC851.8189894E-1243.6182863
CCTAACG903.6379788E-1241.2180252
TGGTATC5100.039.67512
GGTATCA15400.039.6641041
GTACGTA953.1106538E-837.8853169
GTATCAA37850.037.0017281
AACTGTG21350.034.3325370
AACGCCA1450.032.530715
ATCAACG43000.032.1373633
GACAGGT3050.032.0876581
TATCAAC43450.031.8998782
TCAACGC43350.031.871974
GGTACGT1001.4439138E-731.81714468
GCCGATG450.003872861631.79570857
AACGCAG44000.031.7781726
CAACGCA43900.031.6970655
ATGTACC1550.030.42488911
CCGATGT450.00489428330.31058533
Produced by FastQC (version 0.11.5)