FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114853
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT39070.35044979024140405No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37660.337802382915057No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33830.30344807790802913No Hit
CTCCTGGACCCCTGCCCGCTTCTCAGGCTCCCTCCTTGGGGGCAAAGCGGCCCTGACACTGTCAGGTGTGCAGC33610.3014747235734218No Hit
GACTATTACTGTCTGCTCTACTATGGTGGTGTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG33340.2990528796173128No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32790.2941194937807944No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32700.2933122124620914No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCCG31000.2780635653310347No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30740.275731419299226No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30440.273040481570216No Hit
GATTTATAGTACAACCAACAAACACTCCTGGACCCCTGCCCGCTTCTCAGGCTCCCTCCTTGGGGGCAAAGCGGC29970.2688246791281003No Hit
CTATTACTGTCTGCTCTACTATGGTGGTGTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC28640.2568948551961559No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA27720.24864264616052523No Hit
CCATAGTAGAGCAGACAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCCGCTTTGCCC27560.24720747937171986No Hit
CAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCCGCTTTGCCCCCAAGGAGGGAGC27170.24370926032400683No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26920.24146681221649852No Hit
CTACTATGGTGGTGTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA26900.2412874163678978No Hit
GTCCAGGAGTGTTTGTTGGTTGTACTATAAATCAGTGGCCTGGGTGCTTG26620.23877587448748847No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA26520.23787889524448513No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACACCACCATAGTAGAGCAGACAGTAATAGTCAG26220.23518795751547514No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25000.2242448107508344No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT24390.21877323736851406No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24050.21572350794230272No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC23040.20666401758796898No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22760.20415247570755965No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21780.19536207912612694No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACACCACCATAGTAGAGCAGACAGTAATAGTCAGC21310.19114627668401124No Hit
ATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGCCACTGATTTATAGTACAACCAACAAACACTC21050.18881413065220257No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19920.17867826520626487No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAACACCAC18920.16970847277623147No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18630.1671072329715218No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAACACTGGAGCAGTCACCAGTGGTCA18220.16342961807520812No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18190.16316052430230712No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17190.15419073187227372No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC17130.15365254432647174No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17120.1535628464021714No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16370.14683550207964638No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAAC16120.144593053972138No Hit
GCCTGAGGACGAGGCTGACTATTACTGTCTGCTCTACTATGGTGGTGTTTATGTCTTCGGAACTGGGACCAAGGT15790.14163302247022702No Hit
CTATAAATCAGTGGCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATAGTGACCACTGGTGACTGCT15670.140556647378623No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14740.13221474041869197No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14520.13024138608408461No Hit
CACCTGTGCTTCCAACACTGGAGCAGTCACCAGTGGTCACTATCCAAACT14190.1272813545821736No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13980.1253976981718666No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13580.12180978119985325No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC13460.12073340610824926No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13270.11902914554654291No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13110.11759397875773757No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12810.11490304102872756No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12740.11427515555862522No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCCGCTTTGCCCCCAAGGAGGGAGCCTGAGAAGCGGGCAGGGGTC12720.11409575971002454No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12540.11248119707261856No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12430.11149451990531488No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11940.10709932161459851No Hit
CCTGACAGTGTCAGGGCCGCTTTGCCCCCAAGGAGGGAGCCTGAGAAGCG11800.10584355067439384No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11770.10557445690149285No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11640.1044083838855885No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11600.10404959218838718No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTG11440.10261442539958182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.048.5412561
CCTAACG350.001429085438.9615972
AATGGTA4550.036.0046770
GTGGTAT2000.035.7991751
TCGTATG807.128074E-834.4811740
GTATCAA23100.033.7992671
CGTATGC851.1958946E-732.5089141
CTAAAGG652.5184057E-531.4689833
TAAAGGG553.492609E-430.9921784
TATCAAC24850.030.867462
TGGTATC2450.030.612682
CGATAGA450.00470638430.55428137
GGCGATA450.004753425730.4922235
TACTACA6050.030.32723870
CCGTCCG450.00490108530.3021039
TCAACGC26650.029.0384524
ATCAACG26700.028.984073
CGAGACG1203.0504452E-928.40822223
CGCAATA500.006821181628.31988357
CAACGCA27500.028.2648685
Produced by FastQC (version 0.11.5)