FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922279
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCCTCAAGCACTTTTGGCCAGGGGA54120.5868072459635316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34160.37038683522014487No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAACTCTGCCCCAGACCCACTGCCA31920.3460991738942337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31020.336340738540073No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26860.2912350817919523No Hit
GTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26210.28418732292505844No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCCTCAAGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25380.27518787698733244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22070.23929852029591914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22000.2385395308794844No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20800.2255282837406034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20250.21956479546861632No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19830.215010858970008No Hit
GTATGGTGGCTCCTCAAGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19440.21078220364987169No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19230.2085052354005675No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18880.2047102883183939No Hit
GTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAACTCTGCCCCAGACCCACTGCCA18870.20460186125890323No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18460.20015635181978553No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18340.19885522710589745No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17960.1947349988452518No Hit
GTCTTTGTCTCCAGCGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17570.1905063435251155No Hit
CCTCATGTATGGTGCATCCAGCAGGGCCTCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGGCAGAGTT17430.18898836469224606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16940.183675438777203No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16650.18053105405197342No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGCGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTAGCAG16080.17435071166100496No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15380.16676081749665775No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGCGGATAGAGCCACCCTCTCCTGC14670.15906249627281982No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14600.1583035068563851No Hit
GGCCAAAAGTGCTTGAGGAGCCACCATACTGCTGACAGTAATACACTGCA14420.15635181978555296No Hit
GAGTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGGCTC14400.15613496566657162No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13920.15093046681101924No Hit
CAGTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13800.14962934209713113No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13640.14789450914528035No Hit
CTCCCAGACTCCTCATGTATGGTGCATCCAGCAGGGCCTCTGGCATCCCA13410.14540068677699483No Hit
GTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC13260.1437742808846347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13130.14236472911125592No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTAGCAGCTACTATTTAGCCTGGTACC13130.14236472911125592No Hit
GAACCATGGAGACCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTC12610.13672652201774083No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12550.1360759596607968No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12530.13585910554181543No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTAGCTG12000.13011247138880966No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11980.12989561726982832No Hit
GAGTGAACTCTGCCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA11940.12946190903186564No Hit
ATACATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTAGCTGCTAAAACTCTGA11670.1265343784256174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11570.12545010783071064No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11070.12002875485617694No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10710.11612538071451264No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10680.1158000995360406No Hit
CTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATGTATGGTGCATCCAGCAGGGCCT10650.11547481835756858No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10430.11308942304877376No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGTTTCTCT10390.11265571481081105No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10380.11254728775132038No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10350.11222200657284834No Hit
GTCTGGGGCAGAGTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT10330.11200515245386701No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10270.11135459009692295No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT10150.11005346538303486No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT10140.10994503832354417No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10100.10951133008558149No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10080.10929447596660014No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTAGCAGCTACTATTTA10070.10918604890710945No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10000.10842705949067474No Hit
GCCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGAGGCCCTGCT9960.10799335125271203No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC9870.10701750771729597No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9860.10690908065780529No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9820.10647537241984259No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9740.10560795594391717No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9590.10398155005155707No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9490.10289727945665032No Hit
CCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC9480.10278885239715964No Hit
CTGCAGGAGAGGGTGGCTCTATCCGCTGGAGACAAAGACAGGGTGCCTGG9470.10268042533766897No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9460.10257199827817831No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9460.10257199827817831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)