FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922279
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCCTCAAGCACTTTTGGCCAGGGGA46560.5048363889885815No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33630.3646402010671391No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAACTCTGCCCCAGACCCACTGCCA29680.32181151256832263No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26130.2833199064491331No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25910.28093451114033824No Hit
GTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25150.27269405461904694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24530.2659715769306251No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCCTCAAGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA23120.25068336154244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22570.2447198732704529No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20780.2253114296216221No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20610.2234681696102806No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19750.21414344249408257No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19240.2086136624600582No Hit
GTAATACACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTGAGAGTGAACTCTGCCCCAGACCCACTGCCA17780.19278331177441965No Hit
GTATGGTGGCTCCTCAAGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17740.19234960353645697No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17030.18465128231261907No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16910.18335015759873097No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16900.1832417305392403No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16900.1832417305392403No Hit
GTCTTTGTCTCCAGCGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16580.17977206463553871No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16530.17922992933808532No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16320.17695296108878117No Hit
CCTCATGTATGGTGCATCCAGCAGGGCCTCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGGCAGAGTT16170.17532655519642104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15570.16882093162698056No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGCGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTAGCAG14030.15212316446541665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13920.15093046681101924No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13910.15082203975152855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13900.15071361269203787No Hit
GGCCAAAAGTGCTTGAGGAGCCACCATACTGCTGACAGTAATACACTGCA13860.15027990445407516No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13680.14832821738324303No Hit
GAGTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGGCTC13560.14702709266935496No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13540.1468102385503736No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGCGGATAGAGCCACCCTCTCCTGC13130.14236472911125592No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12840.13922034438602635No Hit
GTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC12330.13369056435200194No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTAGCAGCTACTATTTAGCCTGGTACC12290.13325685611403926No Hit
GTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTAGCTG11940.12946190903186564No Hit
CAGTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11940.12946190903186564No Hit
CTCCCAGACTCCTCATGTATGGTGCATCCAGCAGGGCCTCTGGCATCCCA11730.12718494078256146No Hit
GAACCATGGAGACCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTC11670.1265343784256174No Hit
GAGTGAACTCTGCCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA11650.12631752430663606No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11050.11981190073719558No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10940.11861920308279816No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10720.11623380777400331No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10690.11590852659553129No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10350.11222200657284834No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10300.11167987127539496No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10270.11135459009692295No Hit
GTCTGGGGCAGAGTTCACTCTCACCATCAGCAGAGTGGAGCCTGAAGATT10180.11037874656150688No Hit
ATACATGAGGAGTCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAATAGTAGCTGCTAAAACTCTGA10140.10994503832354417No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAGACCCCAGCGCAGTTTCTCT10090.10940290302609079No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10020.10864391360965607No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9970.10810177831220272No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTAGCAGCTACTATTTA9730.10549952888442651No Hit
CTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGACTCCTCATGTATGGTGCATCCAGCAGGGCCT9660.10474053946799178No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9580.10387312299206639No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9470.10268042533766897No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9450.10246357121868761No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9420.1021382900402156No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9330.10116244650479951No Hit
CCCCAGCGCAGTTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC9320.10105401944530885No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9240.10018660296938345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC252.8321892E-454.111773
GTATCGG200.00796632450.7215414
TACGTAT605.6526676E-447.03409270
GGTATCA10450.038.888921
CCTAACG350.001481881138.6743352
GTATCAA25350.037.1387941
AACTGTG22350.035.9858170
TGGTACG651.2906994E-534.69727767
GTAGTTT1052.1735615E-433.59577670
GGTACGT602.4041034E-433.02787868
GTACGTA550.003370488532.70928269
ATCAACG28550.032.6945043
TATCAAC28700.032.307222
TCAACGC28900.032.2932934
CAACGCA29450.031.6901975
AACGCAG29650.031.4764336
TGGACTA900.00414350931.35605870
TTCGATC450.004673118730.59803239
GTTCTAC450.005061278630.1029051
ACGCAGA31300.029.9251637
Produced by FastQC (version 0.11.5)