FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171827
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43690.3728366047206627No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCT42560.363193543074191No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATAAGGCGT41610.35508654434485637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40990.34979566096360637No Hit
ATATAATAGTTATACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40950.34945431364868706No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC33510.2859637130736875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32620.2783687353167319No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA32550.27777137751562303No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28980.24730612965907084No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26940.22989741659818386No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25960.22153440738265973No Hit
GTGTATTACTGTGCAAGAGATCGCGGAAACCCCGATGCTTTCAATATCTGGGGCCAAGGGACAATGGTCACCGT25690.21923031300695411No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25160.2147074610842727No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25030.21359808231078478No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24130.20591776772509937No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA23860.2036136733493937No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23390.19960284239909135No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23030.1965307165648172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22660.19337325390181315No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22570.1926052224432446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22530.19226387512832527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21920.18705832857580512No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAA21020.1793780139901197No Hit
CAATATAATAGTTATACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20830.17775661424425276No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCG20520.1751111725536278No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19550.1668335001668335No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19240.16418805847620851No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT18990.16205463775796256No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGA18440.15736111217782145No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17410.14857141881864816No Hit
GTACTAATACTCTGACTGGCCCGGCAAGCGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA17240.1471206927302409No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16750.14293918812247883No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16730.14276851446501915No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16660.14217115666391028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16040.13688027328266034No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT15950.1361122418240918No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15900.1356855576804426No Hit
CTAATACTCTGACTGGCCCGGCAAGCGATGGTGACTCTGTCTCCTACAGA15760.13449084207822487No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCACC15520.13244275818870874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15310.13065068478538214No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15180.12954130601189423No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15050.12843192723840635No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15040.12834659040967653No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC15030.12826125358094667No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14560.1242504226306443No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14490.12365306482953542No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14180.12100762313891043No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14070.1200689180228822No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13800.1177648236471766No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13730.11716746584606771No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13410.11443668732671289No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13400.11435135049798306No Hit
ACTTATTACTGCCAACAATATAATAGTTATACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13400.11435135049798306No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13140.11213259295100728No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13140.11213259295100728No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGAGTATTAGTAC13090.11170590880735809No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13020.11110855100624922No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC12990.1108525405200597No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12990.1108525405200597No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12930.11034051954768066No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12910.110169845890221No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12810.10931647760292261No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12740.10871911980181374No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12690.10829243565816456No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12290.10487896250897102No Hit
GGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT12260.10462295202278152No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA12220.10428160470786216No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12140.10359891007802347No Hit
GTCCATACTGGATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGTG12100.1032575627631041No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12040.10274554179072508No Hit
CCTTATAGATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA12000.10240419447580572No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11990.10231885764707589No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11920.10172149984596703No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11920.10172149984596703No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11820.10086813155866864No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11740.10018543692882993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)