FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995906
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82030.41099129918944083No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC79420.39791453104504926No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63110.31619725578258695No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG54300.2720569004752729No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54020.2706540287969474No Hit
GTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGATACA53670.26890043919904044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53460.26784828544029626No Hit
CTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCA52610.2635895678453795No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA50780.25442079937632334No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGAGCTTTCACAACTATTTAAATTGGTATCAGCAGAAA49100.24600356930637013No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47710.23903931347468269No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47200.23648408291773262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG46990.23543192915898842No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46800.2344799805201247No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGAGCTTTCACAA45470.2278163400480784No Hit
ACTTACTACTGTCAACAGAGTTTCGATTCGCCTCACACTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAA44990.22541141717094892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44780.22435926341220477No Hit
GGCCAAAAGTGTGAGGCGAATCGAAACTCTGTTGACAGTAGTAAGTTGCA44270.22180403285525474No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCTTGATGGGAC44250.2217038277353743No Hit
GGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC43290.21689398198111534No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42740.21413834118440447No Hit
GATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCA42450.21268536694613877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42370.21228454646661718No Hit
CTCCTGATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTT41850.2096792133497269No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTTCGATTCGCCTCA41280.20682336743313565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40640.20361680359696296No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG39730.199057470642405No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAGTAACTACTACATGAGCTGGGTCCGCCAGGCTCCCGGGAAGGGG39240.19660244520533532No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC38880.1947987530474882No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCC38810.19444803512790682No Hit
GTTTAGTCCCCTGGCCAAAAGTGTGAGGCGAATCGAAACTCTGTTGACAG38400.19239383017035872No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT38330.19204311225077736No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTTCGATTCG37420.18748377929621937No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36890.1848283436193889No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCT34740.1740562932322464No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34140.17105013963583457No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33280.16674131948097756No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33030.1654887554824726No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAAGTG32840.16453680684360888No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32610.16338444796498433No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31660.15862470477066556No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30790.15426578205586838No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30650.1535643462167056No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT30370.15216147453838005No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT29570.14815326974316426No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29550.14805306462328385No Hit
GTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTA28880.14469619310729062No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28840.14449578286752982No Hit
ATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCA28750.14404485982806806No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC28720.14389455214824748No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGA28550.1430428086292641No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCTTGATGGGACCCCA28480.14269209070968272No Hit
GTGTATTACTGTGCGAGAGATTACGCCTTAGAGACCAGTGCCTACTACCCGTGCGATTTCTGGGGCCAGGGGACC27730.1389343987141679No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27530.13793234751536396No Hit
AGTCAGAGCTTTCACAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG27290.1367298860767992No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCA26820.13437506575960992No Hit
GTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTTCGATTCGCCTCACACTTTTGGCCAGGGGA26780.13417465551984914No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26640.13347321968068637No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26250.13151921984301865No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26110.13081778400385588No Hit
GTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCACT25900.12976563024511173No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGTGAAAGCTCTGACTTGCCCGGCAAGTGA25700.12876357904630778No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25640.1284629636866666No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25290.12670937408875968No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25060.12555701521013513No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTT24540.12295168209324486No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24450.12250075905378309No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA24380.1221500411342017No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCG24310.12179932321462032No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24220.12134840017515855No Hit
CAGTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGATA24060.12054675921611539No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23710.11879316961820847No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23480.11764081073958393No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23470.11759070817964373No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAAC23350.11698947746036135No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23020.11533609298233485No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22940.11493527250281325No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22800.11423383666365049No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22160.11102727282747785No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22070.11057634978801607No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC22050.11047614466813567No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21930.10987491394885332No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG21930.10987491394885332No Hit
AAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT21870.10957429858921212No Hit
GAGTCACCGTCACTTGCCGGGCAAGTCAGAGCTTTCACAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG21860.10952419602927191No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21830.10937388834945133No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21750.10897306786992975No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21270.10656814499280026No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21250.10646793987291987No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT21170.10606711939339829No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20770.10406301699579038No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20740.1039127093159698No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTCA20290.10165809411866089No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20010.10025522244033536No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19970.10005481220057458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)