FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995906
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81820.4099391454306966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67250.33693971559782876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC64370.32251017833505186No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG56090.28102525870456824No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51050.25577356849470867No Hit
CTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCA49720.2491099280226624No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49690.2489596203428418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49450.24775715890427707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47330.23713541619695516No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGAGCTTTCACAACTATTTAAATTGGTATCAGCAGAAA46870.23483069843970608No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46870.23483069843970608No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46600.23347792932132075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46320.23207505764299524No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCTTGATGGGAC45760.22926931428634412No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44370.22230505845465667No Hit
GTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGATACA44230.2216036226154939No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGAGCTTTCACAA44200.22145331493567336No Hit
GGCCAAAAGTGTGAGGCGAATCGAAACTCTGTTGACAGTAGTAAGTTGCA42730.21408823862446427No Hit
CTCCTGATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTT41990.21038064918888966No Hit
GATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCA41980.21033054662894946No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41660.20872726471086311No Hit
GGACTAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC40650.20366690615690317No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40320.20201352167887668No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTAGTAACTACTACATGAGCTGGGTCCGCCAGGCTCCCGGGAAGGGG40030.20056054744061091No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38830.19454824024778722No Hit
ACTTACTACTGTCAACAGAGTTTCGATTCGCCTCACACTTTTGGCCAGGGGACTAAACTGGAGATCAAACGAA38430.19254413785017932No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38270.19174249689113615No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTTCGATTCGCCTCA37600.18838562537514292No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA37480.18778439465586055No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37240.1865819332172958No Hit
GTTTAGTCCCCTGGCCAAAAGTGTGAGGCGAATCGAAACTCTGTTGACAG37050.18562998457843205No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC36820.1844776256998075No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCC36200.18137126698351524No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTTCGATTCG35660.17866572874674458No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCT35580.178264908267223No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG35000.17535895979069155No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTAGTCCCCTGGCCAAAAGTG34700.1738558829924856No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33990.17029860123673157No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT33970.1701983961168512No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33780.16924644747798742No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT33280.16674131948097756No Hit
GTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCACT32390.16228219164629998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31340.15702142285257925No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCAAGTCAGA29450.1475520390238819No Hit
GTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTA29070.14564814174615437No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28850.14454588542747004No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC28660.14359393678860627No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28490.14274219326962292No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28440.14249168046992194No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28370.14214096255034056No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCTCTGAACCTTGATGGGACCCCA27920.13988634735303165No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27510.13783214239548355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27490.13773193727560318No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27330.13693029631656No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT27230.13642927071715802No Hit
ATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTTCAGAGGCAGTGGATCTGGGACAGATTTCACTCTCA27000.13527691183853346No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26710.13382393760026776No Hit
GTGTATTACTGTGCGAGAGATTACGCCTTAGAGACCAGTGCCTACTACCCGTGCGATTTCTGGGGCCAGGGGACC26220.13136891216319807No Hit
GTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTTCGATTCGCCTCACACTTTTGGCCAGGGGA26130.13091798912373628No Hit
CCCCTAAACTCCTGATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCA25490.12771142528756363No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGTGAAAGCTCTGACTTGCCCGGCAAGTGA25420.12736070736798227No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT25400.12726050224810187No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25350.1270099894484009No Hit
AGTCAGAGCTTTCACAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG25150.1260079382495969No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24880.1246551691312116No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24860.1245549640113312No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24590.12320219489294587No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTCACTTGCCG23790.11919399009773005No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA23160.11603752882149762No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23080.11563670834197602No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATCCTATTTGCAAAGTGGGGTCCCATCAAGGTT22970.11508558018263385No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22860.11453445202329168No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22350.11197922146634161No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT22320.111828913786521No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22270.11157840098682004No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21750.10897306786992975No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21670.10857224739040816No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21620.10832173459070717No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAAC21610.10827163203076698No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21410.10726958083196303No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20450.10245973507770406No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20350.10195870947830209No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCGTCA20210.10125727363913932No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20120.10080635059967753No Hit
CAGTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGATA20050.10045563268009615No Hit
GAGTCACCGTCACTTGCCGGGCAAGTCAGAGCTTTCACAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG19980.10010491476051477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC306.7814183E-445.3359873
GTGGTAT5000.044.929911
GGTTACG350.00135617739.380439
CCTAACG350.001444715938.8769532
GTATCAA44850.033.240941
GCTTGTA1057.4396667E-1032.416961
TATCAAC48450.030.3312452
ATCAACG49600.029.6831913
TCAACGC49600.029.680964
CAACGCA50450.029.2482765
AACGCAG50600.029.1615726
CGTACCA2200.027.81632614
ATTCACT5050.027.6180342
GTGCTAG2850.027.469111
CGGAAAT500.00809596727.344130
ACGCAGA54300.027.2371187
AATACGT2250.027.19818510
ATACGTA2250.027.19750411
CGCAGAG55050.026.804288
TCTGGAG20100.026.75545370
Produced by FastQC (version 0.11.5)