FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCTGTCACAGAGAGAAGCCTCAGGGAGA56920.4136955616396938No Hit
GTAATATACTGCAAGATCTTCTGGCTCCAGTCTGAAGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA40650.295444915331229No Hit
GTATATTACTGTCAACAGTATGATTACTTCTATACTTTTGGCCAGGGGAC39920.2901392624851823No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32310.2348296485695451No Hit
GTGTATTACTGTGCGAGACATCAGGGTATACCAGCATCTGCTACTATGGG29950.21767712703985997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29300.21295291560159924No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC27020.1963818354797001No Hit
GTCTCTAGTGGCTCCGTCAGCAGTGGTAGTCACCACTGGGGCTGGCTTCG25750.18715145313109832No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25190.18308136327659677No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24720.17966539500585438No Hit
ATCTACTACAACCCGTCGCTCCAGAGTCGAGTCACCATATCCGGAGACAT23980.17428706198383448No Hit
CAGTAATACACAGCCGTGTCTGCGGCTGTCACAGAGAGAAGCCTCAGGGA23790.17290613864034288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23200.16861800825792161No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22180.1612046303086509No Hit
CTCCTGCAGGGCCAGTCAGCGTGTTGGCATCAGCTACTTAGCCTGGTACC21180.1539366127113267No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTGTTGGCAT21110.153427851479514No Hit
GTATTACTGTGCGAGACATCAGGGTATACCAGCATCTGCTACTATGGGTG20480.14884900039319976No Hit
GTATAGAAGTAATCATACTGTTGACAGTAATATACTGCAAGATCTTCTGG20390.14819487880944057No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20240.14710467616984194No Hit
ATATTACTGTCAACAGTATGATTACTTCTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG20240.14710467616984194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20070.14586911317829682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19440.14129026209198256No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19300.14027273962835718No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19300.14027273962835718No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19270.14005469910043747No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18990.13801965417318668No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18440.13402224449465838No Hit
GGGTATACCAGCATCTGCTACTATGGGTGCTTTTGATATCTGGGGCCAAG18420.1338768841427119No Hit
CACCTGCACTGTCTCTAGTGGCTCCGTCAGCAGTGGTAGTCACCACTGGG18390.13365884361479216No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18240.13256864097519352No Hit
GGTATACCCTGATGTCTCGCACAGTAATACACAGCCGTGTCTGCGGCTGTCACAGAGAGAAGCCTCAGGGAGAA17690.12857123129666523No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17670.12842587094471875No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17630.12813515024082575No Hit
CTTCTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG17320.12588206478565525No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17300.12573670443370877No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16970.12333825862659178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16880.1226841370428326No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16690.12130321369934101No Hit
CAGTAATATACTGCAAGATCTTCTGGCTCCAGTCTGAAGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA16670.12115785334739451No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16320.11861404718833105No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16320.11861404718833105No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16150.11737848419678594No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16060.11672436261302675No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16030.11650632208510703No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15860.11527075909356192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15850.11519807891758867No Hit
GAGTGAAGTCTGTGCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA15560.11309035381436465No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG15350.11156407011892658No Hit
CTCCAGAGTCGAGTCACCATATCCGGAGACATTTCCGGAGACACGTCCAAGAACCAGTTCTCCCTGAGGCTTCT15170.1102558269514082No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15130.10996510624751525No Hit
CCACTGGGGCTGGCTTCGCCAGCCCCCCGGGAAGGGGCTGGAGTGGATTGGAAGTATCTATAAAAGTGGGACCAT14950.10865686307999689No Hit
GACTTCACTCTCACCATCTTCAGACTGGAGCCAGAAGATCTTGCAGTATATTACTGTCAACAGTATGATTACTT14900.10829346220013068No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14790.10749398026442501No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14780.10742130008845178No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14730.10705789920858558No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14320.10407801199368263No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14260.1036419309378432No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14020.10189760671448538No Hit
GTAGTAGATGGTCCCACTTTTATAGATACTTCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGAAGCCAGCC13900.10102544460280646No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13760.10000792213918108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)