FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375891
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCTGTCACAGAGAGAAGCCTCAGGGAGA48890.35533338033318046No Hit
GTAATATACTGCAAGATCTTCTGGCTCCAGTCTGAAGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA38130.277129510985972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37390.2717511779639521No Hit
GTATATTACTGTCAACAGTATGATTACTTCTATACTTTTGGCCAGGGGAC34780.2527816520349359No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32510.23628325208900997No Hit
GTGTATTACTGTGCGAGACATCAGGGTATACCAGCATCTGCTACTATGGG28850.20968230768280338No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC25810.18758753418693777No Hit
GTCTCTAGTGGCTCCGTCAGCAGTGGTAGTCACCACTGGGGCTGGCTTCG25650.1864246513713659No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24640.17908395359806845No Hit
ATCTACTACAACCCGTCGCTCCAGAGTCGAGTCACCATATCCGGAGACAT23440.1703623324812794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23170.16839996773000188No Hit
CAGTAATACACAGCCGTGTCTGCGGCTGTCACAGAGAGAAGCCTCAGGGA21110.153427851479514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19860.14434282948285873No Hit
GTATTACTGTGCGAGACATCAGGGTATACCAGCATCTGCTACTATGGGTG19830.14412478895493902No Hit
GTATAGAAGTAATCATACTGTTGACAGTAATATACTGCAAGATCTTCTGG19810.14397942860299254No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19650.14281654578742065No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19530.14194438367574175No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19120.13896449646083883No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18970.1378742938212402No Hit
CTCCTGCAGGGCCAGTCAGCGTGTTGGCATCAGCTACTTAGCCTGGTACC18960.13780161364526697No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18920.13751089294137397No Hit
ATATTACTGTCAACAGTATGATTACTTCTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG18560.13489440660633728No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTGTTGGCAT18200.13227792027130056No Hit
GGTATACCCTGATGTCTCGCACAGTAATACACAGCCGTGTCTGCGGCTGTCACAGAGAGAAGCCTCAGGGAGAA18130.13176915903948785No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18040.1311150374557287No Hit
CACCTGCACTGTCTCTAGTGGCTCCGTCAGCAGTGGTAGTCACCACTGGG17560.12762638900901307No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17000.12355629915451151No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16990.12348361897853827No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16780.12195733528310018No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16600.12064909211558182No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16600.12064909211558182No Hit
GGGTATACCAGCATCTGCTACTATGGGTGCTTTTGATATCTGGGGCCAAG16570.1204310515876621No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15740.11439859698188302No Hit
CAGTAATATACTGCAAGATCTTCTGGCTCCAGTCTGAAGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA15700.11410787627799004No Hit
CTTCTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG15530.11287231328644494No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15440.11221819170268575No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15380.11178211064684629No Hit
GACTTCACTCTCACCATCTTCAGACTGGAGCCAGAAGATCTTGCAGTATATTACTGTCAACAGTATGATTACTT15300.11120066923906036No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGG14870.10807542167221096No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14820.10771202079234474No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14730.10705789920858558No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14650.10647645780079962No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14630.10633109744885313No Hit
CTCCAGAGTCGAGTCACCATATCCGGAGACATTTCCGGAGACACGTCCAAGAACCAGTTCTCCCTGAGGCTTCT14530.10560429568912072No Hit
GTAGTAGATGGTCCCACTTTTATAGATACTTCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGAAGCCAGCC14410.10473213357744182No Hit
GAGTGAAGTCTGTGCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA14280.10378729128978967No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14240.1034965705858967No Hit
CCACTGGGGCTGGCTTCGCCAGCCCCCCGGGAAGGGGCTGGAGTGGATTGGAAGTATCTATAAAAGTGGGACCAT14120.1026244084742178No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13960.10146152565864593No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13950.10138884548267267No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13830.10051668337099377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACG200.006710037652.9729154
GTCGCTA200.007216732552.00668343
CTAACGC352.5400352E-548.3688433
TTCGCAC306.91259E-445.1590317
GGTATCA13100.044.5003281
CCTAACG405.5766675E-542.338132
TGGTATC4400.036.949642
GTATCAA31400.034.2165031
GCGGTTG553.6232852E-430.79920620
TATCAAC35000.030.4834562
GTCCTAA3151.8189894E-1230.07506270
ATCAACG35650.030.0117473
TCAACGC35950.029.6595764
CAACGCA36500.029.3053915
AACGCAG37100.028.8314486
CGCAGAG39350.027.1819028
ACGCAGA39250.027.1659037
ACGGGTC1005.898801E-727.09443516
GCAGAGT39800.026.9596149
AGAGTAC39600.026.92482411
Produced by FastQC (version 0.11.5)