FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669875
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGAACTGTGGGTGCACGATGATCCTGCTGACCGGCAGCAGCTGGTC27150.16258701998652592No Hit
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGGAGCCGGTGAAGGTCTCCAGCCACGT25210.15096938393592335No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT24050.14402275619432592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC1500.045.220943
TCGTATG5150.044.3208340
CGTATGC5700.043.77956441
CTCGACG6150.043.68162528
CCTAACG1750.042.6368872
TCGACGT6600.041.24704729
ACTCGAC6750.040.28203227
CTCGTAT4650.037.83690639
CGCGTAT557.3987467E-637.6345739
ACGAGAC7450.036.8836322
TCCCGCA12450.035.6532970
GACTCGA7750.035.501626
TAACGCC1950.034.785344
TATGCCG7250.034.55408543
GTATGCC7450.034.5001242
ATCGCGT601.3753221E-534.3800237
GTCGTAT1650.033.4529539
ACGTCAT8050.032.64443632
AGGGGGG9100.031.84145770
ATCTCGT5400.031.83335137
Produced by FastQC (version 0.11.5)