FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669875
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71140.42601991167003517No Hit
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGGAGCCGGTGAAGGTCTCCAGCCACGT25540.152945579758964No Hit
GTGTAGAACTGTGGGTGCACGATGATCCTGCTGACCGGCAGCAGCTGGTC24090.1442622950819672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54750.033.3572241
GGTATCA26300.030.533591
TCCCGCA10750.029.7784670
TATCAAC63250.028.7552322
ATCAACG63200.028.6630733
AACGCAG64150.028.4454696
CAACGCA64700.028.1513355
TCAACGC64300.028.118384
AGAGTAC65650.027.33223311
ACGCAGA66850.027.1953037
CGCAGAG67000.026.982838
CAGAGTA67150.026.82252310
CCTAACG1307.683411E-926.0531222
GCAGAGT71150.025.314589
GCCACGT9850.025.06585569
CTAACGC1401.6945705E-824.1856633
AGTACGG48550.023.42923213
TACACCG9550.023.3970765
GAGTACG49250.023.23509612
ACACCGC10000.022.6827566
Produced by FastQC (version 0.11.5)