FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941036
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43820.46565699930714655No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGCCGGTTGTGTATGAG36600.3889330482574524No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36070.38330095766793193No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG35790.3803255135829023No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTG34250.36396057111523894No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31930.33930689155356436No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT31200.3315494837604513No Hit
TCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCTCATAC30660.3258111273107511No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30600.3251735321496733No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27090.2878742152266226No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGC26200.27841655367063534No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25060.2663022456101573No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24920.26481452356764246No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC24570.26109521846135536No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACA24430.2596074964188405No Hit
CCCAAAGAGTACTGCCGGTTGTGTATGAGCTGCAATAATAGTCAGCCTCG23960.2546130009903978No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23730.25216888620626626No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23500.24972477142213476No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23310.24770572007872174No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22390.23792926094219563No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22080.23463501927662705No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21810.23176584105177697No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGGCAGAGAAG21800.23165957519159733No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21430.22772773836495097No Hit
GCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGAGATGGTCAGGG21210.22538988944099908No Hit
CTATTATTGCAGCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGGCG21050.22368963567812494No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20190.2145507717026766No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20130.21391317654159883No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20120.2138069106814192No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20090.2134881131008803No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19790.21030013729549135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19730.20966254213441357No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19720.20955627627423393No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17910.19032215558172058No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17060.18128955746645187No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16860.17916424026285924No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16840.17895170854249998No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC16280.1730008203724406No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15960.16960031284669236No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15920.16917524940597384No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15550.16524341257932745No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15530.1650308808589682No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15220.16173663919339962No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC15140.16088651231196255No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14960.15897372682872918No Hit
GTGTATGAGCTGCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGA14780.1570609413454958No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGC14690.15610454860387912No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14450.15355416795956797No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14020.1489847359718438No Hit
GTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGCCCCCAAA13970.14845340667094564No Hit
CAATTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT13850.14717821634879005No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13210.14037720129729364No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12860.13665789619100652No Hit
GACCATCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCT12820.13623283275028797No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTA12660.13453257898741386No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12610.13400124968651572No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12530.13315112280507865No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12430.13208846420328235No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11900.12645637361376186No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11830.12571251259250443No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11810.12549998087214517No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11760.12496865157124702No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC11660.12390599296945069No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11610.12337466366855253No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11500.12220573920657658No Hit
GACTTAGAGGCAGAGAAGCGATCAGAAACTCCTGACTGCCGATTATCGAC11450.12167440990567843No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG11310.12018668786316358No Hit
CTCCTGATTTATGAGGTCGATAATCGGCAGTCAGGAGTTTCTGATCGCTT10970.1165736486170561No Hit
CAACAACACCCGGGCAAAGCCCCCAAACTCCTGATTTATGAGGTCGATAATCGGCAGTCAGGAGTTTCTGATCG10950.11636111689669684No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10930.11614858517633757No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10870.11551099001525977No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10720.1139170021125653No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA10710.11381073625238566No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10670.11338567281166714No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10660.11327940695148751No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10450.11104782388771525No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10420.11072902630717635No Hit
AATCAGGAGTTTGGGGGCTTTGCCCGGGTGTTGTTGGTACCAGGAGACACAATTATAACCACCAATGTCACTG10410.11062276044699672No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC10120.10754105050178739No Hit
GACTATTATTGCAGCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGG10070.10700972120088924No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT9940.10562826501855402No Hit
GTACTGCCGGTTGTGTATGAGCTGCAATAATAGTCAGCCTCGTCCTCAAC9920.10541573329819476No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9880.10499066985747622No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGGCAGAGAAGCGATC9820.10435307469639843No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9760.10371547953532065No Hit
GCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG9710.1031841502344225No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9700.10307788437424285No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC9650.1025465550733447No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9600.10201522577244654No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG9550.10148389647154839No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9520.10116509889100948No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9490.10084630131047059No Hit
CTTCTCTGCCTCTAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGC9450.10042123786975206No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9440.10031497200957241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)