FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941036
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39620.4210253380317012No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37880.4025350783604453No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGCCGGTTGTGTATGAG34340.3649169638568556No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG33930.3605600635894908No Hit
TCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCTCATAC29130.3095524507032675No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28420.3020075746305136No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT27780.2952065595790172No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTG27290.2899995324302152No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27190.28893687382841887No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25790.27405965340327043No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25390.26980901899608517No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24590.2613077501817146No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24550.2608826867409961No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGC24440.25971376227902015No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24130.2564195206134516No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC23670.2515312910451885No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22940.24377388325207539No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22780.24207362948920125No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACA22690.2411172367475846No Hit
CCCAAAGAGTACTGCCGGTTGTGTATGAGCTGCAATAATAGTCAGCCTCG21620.22974678970836396No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGGCAGAGAAG21000.2231583063772268No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20780.22082045745327492No Hit
CTATTATTGCAGCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGGCG20360.21635729132573034No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20210.2147633034230359No Hit
GCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGAGATGGTCAGGG19280.20488057842633015No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19270.2047743125661505No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC19240.20445551498561162No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18220.19361639724728916No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18210.1935101313871095No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18060.19191614348441505No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17880.19000335800118168No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17780.18894069939938535No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17650.18755924321705014No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17510.1860715211745353No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16420.17448854241495543No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC15720.1670499322023812No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15610.16588100774040526No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15510.16481834913860893No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15400.163649424676633No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14970.15907999268890882No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14880.15812359994729214No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14740.1566358779047773No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14630.15546695344280134No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14620.1553606875826217No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14530.154404294841005No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14400.1530228386586698No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14210.1510037873152568No Hit
GTGTATGAGCTGCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGA13650.14505289914519742No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13510.14356517710268257No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13430.14271505022124553No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13030.13846441581406027No Hit
GTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGCCCCCAAA12950.1376142889326232No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGC12640.1343200472670546No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12190.12953808355897117No Hit
GACCATCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCT12190.12953808355897117No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12110.1286879566775341No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11830.12571251259250443No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTA11740.12475611985088775No Hit
CAATTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT11730.1246498539907081No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11620.12348092952873216No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11560.12284333436765438No Hit
CTCCTGATTTATGAGGTCGATAATCGGCAGTCAGGAGTTTCTGATCGCTT11520.12241827092693584No Hit
GACTTAGAGGCAGAGAAGCGATCAGAAACTCCTGACTGCCGATTATCGAC11100.11795510479939131No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10970.1165736486170561No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10870.11551099001525977No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC10790.11466086313382273No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10760.11434206555328383No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10520.11179168490897268No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10320.10966636770538003No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC10320.10966636770538003No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG10290.10934757012484113No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA10170.10807237980268555No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9990.10615959431945218No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACC9930.1055219991583744No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9900.1052032015778355No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9770.10382174539550028No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT9590.10190895991226692No Hit
AATCAGGAGTTTGGGGGCTTTGCCCGGGTGTTGTTGGTACCAGGAGACACAATTATAACCACCAATGTCACTG9580.10180269405208728No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9580.10180269405208728No Hit
CTTCTCTGCCTCTAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGC9550.10148389647154839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG200.00632274153.77375862
GTGGTAT3400.047.0567741
GGTATCA10150.042.2578851
TGGTATC3850.040.6529852
GTAACGG350.001182720940.4895457
TAGACTA350.001447305238.8606645
GTATCAA25500.036.043491
TGTAACG501.6072608E-435.37155556
GTTAGTT400.002379172535.11916451
GGTACGT551.7021572E-435.02764568
ATTCCGG2003.5652192E-1034.7057970
GCTTATA502.0135104E-434.0410731
CATACAG502.0267632E-434.0030825
GTACGTA652.1739908E-433.59830569
AATGGTA6100.032.9989570
TATCAAC27600.032.5454442
ATACACC652.2786187E-531.93219441
ATCAACG28700.031.5200773
TCAACGC28750.031.341974
CAACGCA29300.030.7536415
Produced by FastQC (version 0.11.5)