FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830945
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24870.2992977874588571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24850.2990570976418415No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17020.20482703428024718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15900.19134840452737545No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15580.18749736745512638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15480.18629391837004858No Hit
GTGTTTATCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATGAGACGA15070.18135977712122944No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14660.17642563587241034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14620.1759442562383792No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14500.1745001173362858No Hit
ATAGTAAACAGCCGTGTCTGTGGCGGTCACAGAACTCAGTTTCAGGGAGA14260.171611839532099No Hit
GTCTCTGGTGACTCCATCAGCAACCGTTATCTCTATTGGGGCTGGATCCG14100.16968632099597444No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14080.1694456311789589No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14030.16884390663642No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13830.1664370084662643No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13820.16631666355775654No Hit
GTTAATAACTGGCTGGCCTGGTTTCAGCAGAAAGCAGGCCAAGCCCCAAA13260.15957734868132067No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13240.1593366588643051No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13120.15789251996221168No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12580.1513938949027914No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTATTACTGCCAGCAATATAGTGTTTAT12560.15115320508577584No Hit
GATAAACACTATATTGCTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT12210.14694113328800343No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12120.14585802911143336No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11940.1436918207582933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11850.14260871658172322No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAACCGTTATCTCTATTGGG11660.14032216332007533No Hit
GTTTACTATTGTGCGAGGCTTCACGTTGGACTCCCTGGTGGCTGGGTGCC11490.13827629987544302No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11340.13647112624782626No Hit
GTAGGGGACAGAGTCATCATCACTTGCCGGGCCAGTCAGGGTGTTAATAA11320.1362304364308107No Hit
ATATAGTGTTTATCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATGAGACGAACTGTGGCTGCACCAT11230.13514733225424067No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT11120.13382353826065502No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10910.1312962951819916No Hit
GCAATATAGTGTTTATCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATGAGACGAACTGTGGCTGCACCAT10880.13093526045646822No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGGGACAGAGTCATCATCACTTGCCGGGCCAGTCAGGGTGTTAATAACTGG10790.12985215627989818No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10690.12864870719482036No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10390.12503835993958684No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10320.12419594558003236No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10050.12094663305032222No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10010.12046525341629108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9970.11998387378225996No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9850.11853973488016656No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9810.11805835524613542No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9630.11589214689299533No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9490.11420731817388635No Hit
GTCTGCGTCTGTAGGGGACAGAGTCATCATCACTTGCCGGGCCAGTCAGG9480.11408697326537859No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9360.11264283436328518No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9220.11095800564417621No Hit
ACACTATATTGCTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC9190.11059697091865285No Hit
CTATTGGGGCTGGATCCGCCAGTCCCCCCAGAGGGGACTGGAGTGGATTGGCAATATCTACTCTGATGGGACCAG9150.11011559128462171No Hit
GGACCAAGGTGGAAATGAGACGAACTGTGGCTGCACCATCTGTCTCCATC9140.10999524637611395No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9140.10999524637611395No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9060.10903248710805166No Hit
GTCTCCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8980.10806972783998942No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8870.10674593384640381No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8820.10614420930386487No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8810.10602386439535708No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8530.10265420695713917No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8440.10157110278056912No Hit
TATCTACTCTGATGGGACCAGCCATTACAACGCGCCCTTTCAGAGTCGAG8430.10145075787206133No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8400.10108972314653798No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8310.10000661896996793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)