FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830945
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25320.30471330834170735No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24870.2992977874588571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22340.2688505256063879No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18320.220471872386259No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15860.1908670248933443No Hit
GTGTTTATCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATGAGACGA15480.18629391837004858No Hit
GTCTCTGGTGACTCCATCAGCAACCGTTATCTCTATTGGGGCTGGATCCG15030.1808783974871983No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14590.17558322151285585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14450.1738983927937469No Hit
ATAGTAAACAGCCGTGTCTGTGGCGGTCACAGAACTCAGTTTCAGGGAGA13960.1680014922768655No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13340.16054010794938292No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12780.15380079307294706No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12710.1529583787133926No Hit
GTTAATAACTGGCTGGCCTGGTTTCAGCAGAAAGCAGGCCAAGCCCCAAA12690.15271768889637702No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12660.15235665417085367No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12560.15115320508577584No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12490.15031079072622136No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12260.14754285783054233No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12210.14694113328800343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12150.1462190638369567No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACTTTGCAACTTATTACTGCCAGCAATATAGTGTTTAT12060.14513595966038667No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11860.14272906149023099No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11770.14164595731366095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11250.13538802207125622No Hit
GTTTACTATTGTGCGAGGCTTCACGTTGGACTCCCTGGTGGCTGGGTGCC11200.13478629752871732No Hit
GATAAACACTATATTGCTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT10790.12985215627989818No Hit
GTAGGGGACAGAGTCATCATCACTTGCCGGGCCAGTCAGGGTGTTAATAA10760.12949112155437484No Hit
ATATAGTGTTTATCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATGAGACGAACTGTGGCTGCACCAT10660.128287672469297No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAACCGTTATCTCTATTGGG10550.1269638784757114No Hit
GCAATATAGTGTTTATCCGTGGACGTTCGGCCACGGGACCAAGGTGGAAATGAGACGAACTGTGGCTGCACCAT10530.12672318865869583No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10420.12539939466511021No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10380.12491801503107906No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10250.1233535312204779No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9880.11890076960568992No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9740.11721594088658094No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9580.1152904223504564No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9540.11480904271642528No Hit
GTCTGCGTCTGTAGGGGACAGAGTCATCATCACTTGCCGGGCCAGTCAGG9540.11480904271642528No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGGGACAGAGTCATCATCACTTGCCGGGCCAGTCAGGGTGTTAATAACTGG9350.1125224894547774No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9270.11155973018671514No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9180.11047662601014506No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9150.11011559128462171No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8970.10794938293148162No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8870.10674593384640381No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8710.10482041531027926No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8690.10457972549326369No Hit
GGACCAAGGTGGAAATGAGACGAACTGTGGCTGCACCATCTGTCTCCATC8660.10421869076774033No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG8650.10409834585923257No Hit
ACACTATATTGCTGGCAGTAATAAGTTGCAAAGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC8630.10385765604221699No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8620.10373731113370922No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8500.10229317223161583No Hit
CTATTGGGGCTGGATCCGCCAGTCCCCCCAGAGGGGACTGGAGTGGATTGGCAATATCTACTCTGATGGGACCAG8450.1016914476890769No Hit
GTCTCCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8370.10072868842101464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)