FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362664
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT17470.4817131008316238No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9360.25809013301568395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.22086559459996027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.2032184060176913No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA7050.19439481172655682No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT6910.19053448922418545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.18364105618423665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.16296075706439017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.14669225509011094No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5110.14090177133655393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.140626034014956No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA5010.1381443981205744No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT4820.13290538901021332No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT4710.12987227847263583No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT4650.1282178545430481No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA4590.1265634306134604No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA4510.12435753204067677No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACT4280.11801557364392386No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC4280.11801557364392386No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT4240.11691262435753204No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT4030.11112214060397503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.11084640328237708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3900.10753755542320163No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGA3830.10560739417201598No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.10505591952882007No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.1042287075640262No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.10395297024242825No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA3720.10257428363443849No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC3700.10202280899124258No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC3640.10036838506165485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAG200.001488197877.445969
CATGCTG200.00258406667.39162466
TGTCAAT200.003058245264.5879762
AGGGGGG4750.062.89700370
AAGTACG800.061.688881
CTTTTCG407.761941E-659.0679868
TTGCCGT251.9520412E-458.33281744
GCCGTGT200.005112194556.7316448
ATGCTGC250.00583612754.86147367
GCACCTC250.00583612754.86147367
GAGTCAC200.00701030252.3806240
GCCGTCT4000.052.0686646
CGGGTCA301.7703715E-551.40030717
AGAGCCG353.6643152E-451.34666
CTGCTTG3950.049.55829254
CTTGAAA4300.049.5241357
TCTTCTG3750.049.38858450
CCGTCTT4250.049.3837147
ATACATG254.4583046E-449.351111
GAGCTAG200.00895515849.2351734
Produced by FastQC (version 0.11.5)