FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362664
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44711.2328215648644476No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9430.26002029426686957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.24292458032779654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.22279575585114597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.2084574151280524No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT6770.18667416672181414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.1676482915315554No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA6040.16654534224516357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5600.15441290009485364No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA5290.1458650431253171No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT4940.13621423686938874No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4560.12573621864866652No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT4530.12490900668387268No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT4370.12049720953830542No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.11911852293031568No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA4310.11884278560871772No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACT4250.11718836167913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.11691262435753204No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.11636114971433614No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA4210.11608541239273819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.11443098846315046No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGTA4100.11305230185516069No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.1119493525687689No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3990.11001919131758323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.10946771667438732No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT3930.1083647673879955No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGA3910.10781329274479959No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC3890.10726181810160368No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA3750.10340149559923235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)