FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032502
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38630.37413971110951844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32290.3127354716988442No Hit
GAGTTACACTACCCCTCCGAGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29000.2808711266418854No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26310.2548179083430347No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23250.22518116187668402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22340.2163676196268869No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22070.21375261258573833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19970.19341366893236042No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTATTGATGGTGAGAGTGAAATCTGTC19650.19031440132803618No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19170.1856654999215498No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18880.18285678865513091No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18720.1813071548529688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17810.17249361260317173No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17750.17191249992736093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17540.1698786055620231No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACTTACTTAAATTGGTATCAGGAGAGA17370.16823211964722587No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17190.16648878161979347No Hit
GATCTATTCTGCATCCAATCTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTCA17020.16484229570499623No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16670.16145247176276656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16660.16135561965013143No Hit
CCATCAATAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAG15910.15409171120249646No Hit
CTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTTCCACTGAACCT15820.15322004218878027No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14650.1418883450104697No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14350.13898278163141572No Hit
GTCTTCAGGTTGCAGACTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC14160.13714259149134822No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13820.13384961966175368No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13730.1329779506480375No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCA13640.13210628163432128No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13510.13084720417006454No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13230.12813534501628082No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC12560.12164625346972693No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACACTACCCCTCC12520.12125884501918639No Hit
GAATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTCCTGATACCAATT12320.11932180276648374No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12310.11922495065384861No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12030.11651309150006489No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCTCCGAGGACATTCGGCCA11630.11263900699465958No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11570.11205789431884877No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11540.11176733798094338No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTTCCA11450.11089566896722719No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11350.10992714784087586No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11300.10944288727770018No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10920.10576250699756513No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10760.10421287319540301No Hit
GATTTCACTCTCACCATCAATAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACACTACC10650.10314749995641655No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10600.10266323939324087No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10540.10208212671743008No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10330.1000482323520923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG1750.046.70754627
ACGAGAC1750.046.6328622
CACGAGA1950.041.84797321
ATCTCGT1400.041.7548537
CGCTCAT1900.041.40039432
CTAGCGC2000.040.90682228
TAGCGCT2100.040.61372829
TCGTATG1450.040.4995840
GACTAGC2050.039.8587526
CGAGACT2050.039.81818423
GTATCAA29900.038.4178431
CGTATGC1500.036.90117341
CATCTCG1700.036.36274336
TATCAAC32950.034.7585682
TAACGGC905.4260454E-934.3425936
GCGCTCA2400.034.17035331
ATCAACG34700.033.0056153
TCAACGC34950.032.7695244
CTTATAC2600.032.6778951
CGAGCCC2500.032.6256115
Produced by FastQC (version 0.11.5)