FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032502
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37500.3631954223817484No Hit
GAGTTACACTACCCCTCCGAGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26180.25355883087877795No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26010.2519123449639807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25320.24522954919215653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22720.22004799990702198No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21820.21133130976986No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20890.20232406329479266No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTATTGATGGTGAGAGTGAAATCTGTC18880.18285678865513091No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17600.17045971823783393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16840.1630989576775638No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16460.1594185773974288No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16290.15777209148263152No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16280.15767523936999636No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16050.15544764077938833No Hit
GATCTATTCTGCATCCAATCTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTCA16020.15515708444148293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16010.1550602323288478No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15740.15244522528769922No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15530.1504113309223614No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15360.14876484500756415No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15230.1475057675433074No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACTTACTTAAATTGGTATCAGGAGAGA14880.14411594360107777No Hit
CTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTTCCACTGAACCT14740.14276001402418592No Hit
GTCTTCAGGTTGCAGACTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC14580.14121038022202378No Hit
CCATCAATAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAG14400.13946704219459138No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14250.13801426050506438No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCA14170.13723944360398332No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14110.13665833092817253No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14110.13665833092817253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13870.13433388022492934No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11910.1153508661484433No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC11870.11496345769790275No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11400.11041140840405152No Hit
GAATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTCCTGATACCAATT11140.10789325347553808No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10970.1062467675607408No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10930.10585935911020027No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10910.10566565488493No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10890.10547195065965974No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10880.10537509854702459No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10710.10372861263222735No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACACTACCCCTCC10530.10198527460479495No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10510.10179157037952467No Hit
GATTTCACTCTCACCATCAATAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACACTACC10440.10111360559107876No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCTCCGAGGACATTCGGCCA10430.10101675347844363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAT901.8575502E-646.0064170
GTGGTAT3500.045.5934141
GTATCAA33400.039.340221
TATCAAC36700.035.8674962
ATCAACG38300.034.3607863
TAACGGC905.4496923E-934.32714536
TCAACGC38700.033.7345354
CAACGCA39100.033.474535
AACGCAG39950.033.101816
CGCAGAG43000.030.9116068
AAAATCG900.00461713530.67093870
AGAGTAC43700.030.41203311
ACGCTTA450.00494313830.2489928
ACGCAGA44900.029.603547
GTACGTA600.00555430429.53541269
GCAGAGT45100.029.3970769
TTAACGG1052.4834662E-829.37860935
GCTTATA950.006014028629.05667970
CAGAGTA47350.027.99610510
TTGTATC855.054175E-627.9324653
Produced by FastQC (version 0.11.5)