FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714971
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28280.39554051842662147No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27230.3808546080889994No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19490.2725984690288138No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18950.2650457151408938No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18810.2630875937625442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17400.24336651416630886No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16340.2285407380159475No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15650.2188899969369387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15510.21693187555858912No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15130.2116169746744973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14860.2078405977305373No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14770.20658180541588397No Hit
ATATTACTGTCAACACTGGCACAGTCTCCCGGTCTCTTTCGGCGGGGGGA14190.19846958827700706No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAAGTA13770.1925952241419582No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAAGTACTATTTAAACTGGTATCAGCAGAAA13670.19119656601456564No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13440.1879796523215627No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13210.18476273862855977No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13070.18280461725021013No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13060.18266475143747088No Hit
GTCTCCCGGTCTCTTTCGGCGGGGGGACCAAGGTGGAACTCAAACGAACT12210.17077615735463397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12140.16979709666545914No Hit
GTTCAGGGGAAGTGGATCTGGGACAGATTTTAGTTTCACCATCAGCAGCC11940.166999780410674No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11730.1640625983431496No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAACTAAAATCTGTCCCAGATCCACTTCCC11350.15874769745905778No Hit
GTGCCAGTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC11270.15762877095714373No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10840.15161454100935562No Hit
ACATATTACTGTCAACACTGGCACAGTCTCCCGGTCTCTTTCGGCGGGGGGACCAAGGTGGAACTCAAACGAA10800.1510550777583986No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10730.15007601706922377No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCCCCGCCGAAAGAG10510.1469989691889601No Hit
CACTGGCACAGTCTCCCGGTCTCTTTCGGCGGGGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT10380.14518071362334975No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAACTAAAATCTGTCCCAGATCCACTTCCC10360.14490098199787124No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9980.13958608111377943No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCCCCGCCGAAAGAGACCGGGAGA9960.13930634948830092No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9230.1290961451583351No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9130.12769748703094252No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT9080.12699815796724623No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8870.12406097589972181No Hit
AGTCAGGACATCAAGTACTATTTAAACTGGTATCAGCAGAAACCAGGGAA8860.12392111008698256No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8660.12112379383219739No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC8600.12028459895576185No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8460.11832647757741223No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8410.11762714851371595No Hit
CCCCTGAACCTTGACGGGACCCCAGTTTCCAAACGGGTTGCATCGTAGAT8400.11748728270097669No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8280.11580889294810559No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8220.11496969807167003No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8170.11427036900797374No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8150.11399063738249524No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA8070.11287171088058118No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8040.1124521134423634No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7880.11021426043853527No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7850.10979466300031748No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC7760.10853587068566418No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT7750.1083960048729249No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7710.10783654162196789No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7670.10727707837101086No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7670.10727707837101086No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7620.10657774930731456No Hit
CCGGGAGACTGTGCCAGTGTTGACAGTAATATGTTGCAAAATCTTCAGGC7590.10615815186909679No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7590.10615815186909679No Hit
GATTTTAGTTTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACACTGGCACAGTCTC7550.10559868861813977No Hit
CTATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTACGATGCAACCCGTTTGGAAA7450.10420003049074718No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7430.10392029886526866No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7410.10364056723979015No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG7330.10252164073787608No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7260.10154258004870127No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7180.10042365354678723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)