FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714971
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27110.37917621833612836No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23140.3236494906786429No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19770.27651471178551296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18870.2639267886389798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18750.2622483988861087No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG18540.25931121681858427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.2376320158439993No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15420.21567308324393578No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15110.2113372430490188No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14420.20168650197000998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13770.1925952241419582No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13610.1903573711381301No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13500.18881884719799824No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13460.18825938394704123No Hit
ATATTACTGTCAACACTGGCACAGTCTCCCGGTCTCTTTCGGCGGGGGGA13440.1879796523215627No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAAGTACTATTTAAACTGGTATCAGCAGAAA13190.18448300700308126No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13040.18238501981199237No Hit
GTCTCCCGGTCTCTTTCGGCGGGGGGACCAAGGTGGAACTCAAACGAACT12360.17287414454572284No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAAGTA12350.17273427873298358No Hit
GTTCAGGGGAAGTGGATCTGGGACAGATTTTAGTTTCACCATCAGCAGCC11960.16727951203615252No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11700.1636430009049318No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11570.16182474533932145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11480.16056595302466814No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11310.15818823420810077No Hit
GTGCCAGTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC10850.1517544068220949No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10840.15161454100935562No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10750.1503557486947023No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10660.14909695638004897No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAACTAAAATCTGTCCCAGATCCACTTCCC10530.14727870081443864No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCCCCGCCGAAAGAG10060.14070500761569352No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAACTAAAATCTGTCCCAGATCCACTTCCC9790.13692863067173353No Hit
ACATATTACTGTCAACACTGGCACAGTCTCCCGGTCTCTTTCGGCGGGGGGACCAAGGTGGAACTCAAACGAA9430.13189346141312025No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9160.1281170844691603No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCCCCGCCGAAAGAGACCGGGAGA9140.12783735284368178No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9080.12699815796724623No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9020.12615896309081068No Hit
CCCCTGAACCTTGACGGGACCCCAGTTTCCAAACGGGTTGCATCGTAGAT8890.12434070752520032No Hit
CACTGGCACAGTCTCCCGGTCTCTTTCGGCGGGGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT8840.12364137846150403No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA8780.12280218358506848No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8770.12266231777232922No Hit
CCGGGAGACTGTGCCAGTGTTGACAGTAATATGTTGCAAAATCTTCAGGC8740.12224272033411145No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT8650.12098392801945813No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8290.11594875876084484No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8250.11538929550988782No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8020.1121723818168849No Hit
AGTCAGGACATCAAGTACTATTTAAACTGGTATCAGCAGAAACCAGGGAA7870.11007439462579602No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG7870.11007439462579602No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7770.10867573649840344No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC7770.10867573649840344No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7720.10797640743470714No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC7700.10769667580922862No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7440.10406016467800791No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7380.10322096980157236No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7360.10294123817609385No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7340.10266150655061534No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7340.10266150655061534No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7310.10224190911239757No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7180.10042365354678723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG150.002400993668.6654937
GCTTACG150.002514896767.867551
ATTTACG300.002735006666.439770
CGATCCG251.9342072E-458.45130565
GTATCAA18400.036.7001151
GTAGACC400.002824734233.9100383
GAATACG400.002827641833.9029249
GTGGTAT2350.033.211781
ATACGTA450.00503519230.13382511
AATACGT450.00503519230.13382510
TACGTAC450.00503519230.13382512
AACGCAG22450.030.0520326
ATCAACG22600.029.7087963
TCAACGC22850.029.3775864
CAACGCA23000.029.3333995
TATCAAC23350.029.200442
ACGCAGA23700.028.467017
GCGTGAA500.00762724927.6779141
CGCAGAG24400.027.6503358
AGAGTAC24500.027.5355511
Produced by FastQC (version 0.11.5)