FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754963
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACAGCCTGGTACCAACAGAAGCCAGGACAGGCCCCCTTACTTGTCATGTATGGTAGAAACAACCGGCC85700.4883293835824459No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC64530.3677000597733399No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACAACATGGTTACCA47700.27180060206397516No Hit
GTATAATAGATTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG46750.2663873825260134No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46070.26251265696199866No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC45580.25972057530557624No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACAAC44680.25459226205908614No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44210.2519141429192524No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT43220.2462729983481133No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43100.2455892232485813No Hit
CTCCTGTACAGCTTCTGGATTCGACTTTGGTGATTATGTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG42030.23949222861108754No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36810.20974801178144498No Hit
AGCCAGGACAGGCCCCCTTACTTGTCATGTATGGTAGAAACAACCGGCCC35100.2000042166131138No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34850.19857968515575541No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG33920.19328042813438231No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC33890.19310948435949932No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCAGCT33190.18912079627889591No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31880.1816562514423381No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCAGCTGTGTCTGTGGCCTTGGGA31210.1778385071366177No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30850.17578718183802164No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC30090.17145660620765224No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30060.17128566243276924No Hit
GCCTGGTACCAACAGAAGCCAGGACAGGCCCCCTTACTTGTCATGTATGGTAGAAACAACCGGCCCTCAGGGATC29750.16951924342564487No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29380.1674109368687545No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28960.16501772402039244No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28710.1635931925630341No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28660.16330828627156244No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28310.16131394223126072No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27650.15755317918383466No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27650.15755317918383466No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27270.15538789136864994No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26980.15373543487811422No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA26420.15054448441363152No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26050.14843617785674112No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25650.14615692752496776No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25350.14444748977613772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25210.14364975216001705No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25080.14290899580219069No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24660.14051578295382866No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG24660.14051578295382866No Hit
CTATTACTGTAATTCCCGGGACAGCAGTGGTAACCATGTTGTATTCGGCG24650.14045880169553432No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24560.13994597037088533No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24450.13931917652964765No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23580.13436180705804054No Hit
GACTATTACTGTAATTCCCGGGACAGCAGTGGTAACCATGTTGTATTCGG23480.1337919944750972No Hit
GGGCTGGAGTGGGTGGGTCTCGTTAGACATAAAGTTTATGGTGGGACCAC22910.13054406275232014No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22910.13054406275232014No Hit
GTCCTGGCTTCTGTTGGTACCAGGCTGTATAATAGATTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT22810.1299742501693768No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22770.12974632513619946No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22620.12889160626178442No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22410.1276949998376034No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC22310.12712518725466007No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22010.12541574950583004No Hit
GCTTGGTCCCTCCGCCGAATACAACATGGTTACCACTGCTGTCCCGGGAATTACAGTAATAGTCAGCCTCATCTT21380.12182593023328696No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21030.11983158619298527No Hit
CAGTAATAGTCAGCCTCATCTTCCGTCTGAGCCCCAGTGATGGTCAAGGA20850.11880592354368724No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATAACATAATCAC20650.11766629837780057No Hit
GTAATAGTCAGCCTCATCTTCCGTCTGAGCCCCAGTGATGGTCAAGGAAG20630.11755233586121189No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCGACTTTGGTGATTATGTT20510.11686856076167987No Hit
GTCTGGGGGAGGCCTGGTACAGCCAGGGCGGTCCCTGAGACTCTCCTGTA20380.11612780440385354No Hit
ACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGA20380.11612780440385354No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCAGCTGTGTCTGTGG20320.11578591685408753No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20210.11515912301284985No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA20080.11441836665502349No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19740.11248100387301611No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACAACATGGTTAC19660.11202515380666145No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19650.11196817254836712No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA19560.11145534122371811No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19560.11145534122371811No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19550.11139835996542377No Hit
TCACTGGGGCTCAGACGGAAGATGAGGCTGACTATTACTGTAATTCCCGG19430.11071458486589175No Hit
ACTCAGGACCCAGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAT19420.11065760360759742No Hit
CTTCTGAGCTGACTCAGGACCCAGCTGTGTCTGTGGCCTTGGGACAGACA19420.11065760360759742No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19340.11020175354124276No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC19290.10991684724977109No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAATCTATTATACAGCCTGGTA18810.10718174685164301No Hit
GGTACAGCCAGGGCGGTCCCTGAGACTCTCCTGTACAGCTTCTGGATTCG18740.10678287804358269No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA18580.10587117791087335No Hit
GGACCCAGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCC18550.10570023413599032No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18390.10478853400328098No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17810.10148362102220959No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17730.1010277709558549No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC17720.10097078969756057No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17650.10057192088950023No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17610.1003439958563229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3900.049.1746370
GCTAATC2750.047.5291533
CGTATGC2800.046.9407641
CGCTAAT2950.046.6147832
TCGCTAA2950.046.5948131
GTGGTAT3600.045.7241971
TCGTATG3000.043.77236640
CTCGTAT2950.043.30552739
ATCTCGT3050.042.95361737
CACTCGC3200.042.89822428
GGTATCA12450.042.694281
TGGTATC3950.041.6631852
ACACTCG3300.041.57930427
ATGCCGT3350.041.40734544
CCGTCTT3400.040.91044247
AATCTCG3300.040.71394736
ACTCGCT3450.039.80214729
CCCACGA3450.039.7431819
GCCGTCT3500.039.70599446
CTCGCTA3500.039.2514730
Produced by FastQC (version 0.11.5)