FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754963
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACAGCCTGGTACCAACAGAAGCCAGGACAGGCCCCCTTACTTGTCATGTATGGTAGAAACAACCGGCC81500.46439725509882546No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC62630.3568736206974164No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT46880.2671281388838397No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45710.2604613316634026No Hit
GTATAATAGATTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG45670.26023340663022526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43350.24701375470593967No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC43220.2462729983481133No Hit
CTCCTGTACAGCTTCTGGATTCGACTTTGGTGATTATGTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG41930.23892241602814418No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACAACATGGTTACCA41400.23590240933854448No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACAAC41290.2352756154973068No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40980.23350919649018242No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT35710.20348007336906818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33160.18894985250401292No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32930.18763928356324322No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCAGCTGTGTCTGTGGCCTTGGGA31970.18216908276698712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31680.1805166262764514No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31510.17954794488544773No Hit
AGCCAGGACAGGCCCCCTTACTTGTCATGTATGGTAGAAACAACCGGCCC31310.17840831971956103No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCAGCT31220.177895488394912No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC30420.17333698773136527No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30170.17191245627400692No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30100.17151358746594658No Hit
GCCTGGTACCAACAGAAGCCAGGACAGGCCCCCTTACTTGTCATGTATGGTAGAAACAACCGGCCCTCAGGGATC29390.16746791812704884No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29220.16649923673604514No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29090.1657584803782188No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28880.16456187395403776No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT28000.15954752322413635No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27810.158464879316544No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC27680.15772412295871766No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26750.15242486593734456No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG26350.15014561560557116No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26110.14877806540650715No Hit
GGGCTGGAGTGGGTGGGTCTCGTTAGACATAAAGTTTATGGTGGGACCAC26010.14820825282356379No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25950.1478663652737978No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25660.1462139087832621No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25000.14245314573583603No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA24400.13903427023817597No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24370.13886332646329294No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24280.13835049513864395No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24100.13732483248934593No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23610.13453275083292354No Hit
CTATTACTGTAATTCCCGGGACAGCAGTGGTAACCATGTTGTATTCGGCG23350.13305123811727085No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22900.1304870814940258No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22860.13025915646084846No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC22500.12820783116225243No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22430.1278089623541921No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA22190.12644141215512805No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21840.12444706811482635No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21700.12364933049870566No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21620.123193480432351No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATAACATAATCAC21560.12285159288258499No Hit
GTCCTGGCTTCTGTTGGTACCAGGCTGTATAATAGATTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT21310.12142706142522663No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21270.12119913639204928No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21170.12062932380910595No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC21070.12005951122616261No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG20930.11926177361004194No Hit
GACTATTACTGTAATTCCCGGGACAGCAGTGGTAACCATGTTGTATTCGG20880.11897686731857024No Hit
GCTTGGTCCCTCCGCCGAATACAACATGGTTACCACTGCTGTCCCGGGAATTACAGTAATAGTCAGCCTCATCTT20650.11766629837780057No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCAGCTGTGTCTGTGG20620.11749535460291755No Hit
ACCATGGAGTTTGGGCTTAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGA20460.1165836544702082No Hit
GTCTGGGGGAGGCCTGGTACAGCCAGGGCGGTCCCTGAGACTCTCCTGTA20050.1142474228801405No Hit
GTGGTAACCATGTTGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCA19940.11362062903890283No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19930.11356364778060848No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT19910.1134496852640198No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19670.11208213506495579No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19430.11071458486589175No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19370.11037269731612576No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA19340.11020175354124276No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAATCTATTATACAGCCTGGTA19280.10985986599147675No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACAACATGGTTAC19170.10923307215023907No Hit
GTAATAGTCAGCCTCATCTTCCGTCTGAGCCCCAGTGATGGTCAAGGAAG19090.10877722208388438No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA19030.10843533453411837No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18910.10775155943458638No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18810.10718174685164301No Hit
CAGTAATAGTCAGCCTCATCTTCCGTCTGAGCCCCAGTGATGGTCAAGGA18800.1071247655933487No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18800.1071247655933487No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCGACTTTGGTGATTATGTT18740.10678287804358269No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18680.10644099049381668No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18640.10621306546063936No Hit
TCACTGGGGCTCAGACGGAAGATGAGGCTGACTATTACTGTAATTCCCGG18360.10461759022839798No Hit
CTTCTGAGCTGACTCAGGACCCAGCTGTGTCTGTGGCCTTGGGACAGACA18280.1041617401620433No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17870.1018255085719756No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT17700.10085682718097191No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17660.10062890214779457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.047.45461
GGTATCA13750.045.613941
TGGTATC4450.041.5645332
GTATCAA29700.035.0805441
GTATACA652.4295072E-531.6364021
CGACTCA450.004791236530.44384418
GGGCAGT5200.029.6591261
TATCAAC34950.029.5970822
ATCAACG36100.028.5512183
TCAACGC36750.028.1394084
CAACGCA36950.027.9847035
AACGCAG37350.027.6850036
AGTGGTG5750.027.3916515
CACGAAC657.2059437E-427.38612664
CGCAGAG39250.026.3448358
AGAGTAC39800.025.80871811
CAGAGTA40350.025.5417810
TTCGGGG18300.025.33882770
TTGACTC851.4709127E-424.28605534
TCGGTTG750.001793528323.42167956
Produced by FastQC (version 0.11.5)