FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051779
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC31930.3035808853380796No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGCCGGTTGTGTATGAG30460.289604565217598No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG27410.2606060778927893No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26490.2518589931915355No Hit
TCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCTCATAC24940.2371220570100753No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24740.23522051685762885No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTG23910.227329125224976No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT23290.22143435075239187No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC21320.20270418025079415No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21260.20213371820506018No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGC20640.1962389437324761No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20600.1958586357019868No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACA20230.19234078641996083No Hit
CCCAAAGAGTACTGCCGGTTGTGTATGAGCTGCAATAATAGTCAGCCTCG20050.19062940028275902No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18770.1784595433071016No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18750.17826938929185693No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18580.17665308016227743No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18380.17475154000983095No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18240.1734204619031184No Hit
CTATTATTGCAGCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGGCG17930.1704730746668264No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17930.1704730746668264No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17650.16781091845340135No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17600.1673355334152897No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGGCAGAGAAG17260.1641029151561307No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17040.1620112209884396No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16830.16001460382837077No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16720.15896875674452524No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16390.15583121549298853No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15920.15136259613473935No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15170.14423182056306505No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14990.14252043442586323No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14960.14223520340299625No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14920.14185489537250695No Hit
GCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGAGATGGTCAGGG14910.14175981836488463No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14620.13900258514383726No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13730.13054073146545045No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC13550.12882934532824863No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13440.12778349824440305No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13430.12768842123678073No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13410.1274982672215361No Hit
GACCATCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCT13280.12626226612244587No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13190.12540657305384495No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12900.12264933983279758No Hit
CTCTACTTGTGCGCTATCGGTCACTGGCCGGTATTTAGCTTTAGAAGACATACACCTCCCATTTAGAGCAGCATT12650.12027241464223949No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC12560.11941672157363857No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12360.11751518142119209No Hit
CAATTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT12100.11504317922301167No Hit
AATCAGGAGTTTGGGGGCTTTGCCCGGGTGTTGTTGGTACCAGGAGACACAATTATAACCACCAATGTCACTG11880.11295148505532056No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11830.11247610001720892No Hit
CTCCTGATTTATGAGGTCGATAATCGGCAGTCAGGAGTTTCTGATCGCTT11660.11085979088762943No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGC11600.11028932884189549No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTA11440.10876809671993833No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11390.10829271168182669No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11300.10743701861322578No Hit
GTGTATGAGCTGCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGA11220.1066764025522472No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11050.1050600934226677No Hit
GACTTAGAGGCAGAGAAGCGATCAGAAACTCCTGACTGCCGATTATCGAC10860.10325363027784355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.041.80291
CGTATGC1250.041.3840541
CGCGTAT557.633105E-637.4619439
GTGGTAT2850.036.7628331
GTATCAA27950.035.914251
TCGTATG1250.035.79151540
TCTCGTA1250.035.6465638
TACGAAT400.002244738635.53834554
TCGCGTA601.4037858E-534.2755438
CACGAGA3150.033.26635721
CCCACGA3150.033.26477419
TATACAC3500.032.832433
AGCATCG1350.032.7879234
ACGAGAC3200.032.74968722
GTATGCC1700.032.5306742
CTCTTAT1253.6379788E-1232.4461671
TGGTATC3150.032.1886562
AGGGGGG2605.456968E-1231.61914370
ATCGCGT652.4610103E-531.57502437
ATCAACG31550.031.1735133
Produced by FastQC (version 0.11.5)