FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051779
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30570.29065041230144356No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28840.27420208998278156No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGCCGGTTGTGTATGAG28390.269923624639777No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27580.2622223870223688No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25790.24520360265797284No Hit
TCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCTCATAC22950.21820173249323288No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22310.21211680400540417No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19980.18996386122940276No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT19940.18958355319891346No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC19010.18074139149003735No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18890.17960046739856947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18850.17922015936808017No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTG18820.17893492834521318No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGC18220.1732303078878738No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACA18160.17265984584213984No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17860.16980753561347012No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17780.16904691955249154No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17350.1649586082247316No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17260.1641029151561307No Hit
CCCAAAGAGTACTGCCGGTTGTGTATGAGCTGCAATAATAGTCAGCCTCG17000.1616309129579503No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16310.15507059943200996No Hit
CTATTATTGCAGCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGGCG15960.15174290416522862No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15480.1471792077993571No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15420.14660874575362315No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGGCAGAGAAG15340.14584812969264457No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15290.14537274465453293No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15110.1436613585173311No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC14920.14185489537250695No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14720.13995335522006047No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14220.1351995048389443No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14030.13339304169412014No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13950.13263242563314157No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13910.13225211760265226No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13490.12825888328251467No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC13070.12426564896237707No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13040.12398041793951012No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12920.12283949384804221No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12870.1223641088099306No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12800.12169856975657434No Hit
GCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGAGATGGTCAGGG12480.11865610551265997No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12160.11561364126874561No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12030.1143776401696554No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12020.11428256316203309No Hit
CTCCTGATTTATGAGGTCGATAATCGGCAGTCAGGAGTTTCTGATCGCTT11980.1139022551315438No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11900.1131416390705652No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11820.11238102300958662No Hit
GACCATCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCT11800.11219086899434197No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11470.10905332774280528No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11310.10753209562084812No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11180.10629609452175791No Hit
GTGTATGAGCTGCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGA10870.10334870728546586No Hit
AATCAGGAGTTTGGGGGCTTTGCCCGGGTGTTGTTGGTACCAGGAGACACAATTATAACCACCAATGTCACTG10530.10011608902630686No Hit
CAATTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT10530.10011608902630686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCC307.0153445E-445.022983
GGTATCA11500.044.086151
GTATCAA27150.035.7289921
TGGTATC2850.034.374362
TTAACTC808.580719E-833.7608155
GTGGTAT3100.032.709081
TATCAAC30250.031.8273832
GGTACGT500.003969935731.63492868
ATCAACG31000.031.0440733
TCAACGC31450.030.5940634
AACGCAG32150.030.0329486
ACATACC450.00513900530.0096138
CAACGCA32100.029.9745565
ACGCAGA33750.028.6091657
CGCAGAG34200.028.3314468
CAGAGTA35200.027.52657710
GCAGAGT35850.027.121669
GCTATCG4600.025.68635713
AGCGCTT3900.025.67136870
TCTACTT4650.025.4270822
Produced by FastQC (version 0.11.5)