FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246074
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53820.4319165635427752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53360.4282249689825805No Hit
GTTTTATATAGCTCCAACAATAAGAACTACATAGCTTGGTACCAGCAGAA49920.40061826183677696No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGAATCAGGGGTCCCT48910.39251280421547996No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA41770.3352128364768064No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37750.3029515101029313No Hit
GCTTAGGAGGCTGTCCTGCCTTCTGCTGGTACCAAGCTATGTAGTTCTTATTGTTGGAGCTATATAAAACACT35460.2845737893576144No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33560.2693258987828973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32870.26378850694260536No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30730.24661456703213452No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30280.24300322452759626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29800.23915112585608883No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29270.2348977669062993No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT29170.23409524634973525No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28310.22719356956328435No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27890.22382298322571534No Hit
ATATAAAACACTCTGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA27510.2207734051107719No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27510.2207734051107719No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25930.2080935803170598No Hit
GTATATTACTGTGCCACACGTGGATACAGCTATGGTTACTTTGACTCCTGGGGCCCGGGTACCCTGGTCACCGT25270.20279694464373707No Hit
GTTTATTACTGTCAGCAATATTATAATACTCTTCCGTACACTTTTGGCCA24690.19814232541566554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24560.19709904869213224No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24030.19284568974234276No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23240.1865057773454867No Hit
TTATAATACTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT23190.18610451706720468No Hit
ATTATAATACTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22810.18305493895226127No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21780.17478897721965148No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21510.17262217171692854No Hit
GAACTACATAGCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGC21250.17053561826986197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21170.16989360182461075No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21150.16973309771329792No Hit
CTACATAGCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGCTCA20970.16828856071148263No Hit
GTCTCTGGGGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG20770.1666835195983545No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20270.16267091681553422No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20060.1609856236467497No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20000.16050411131281128No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACT19990.16042385925715488No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19650.15769528936483707No Hit
AGGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGAA18980.15231840163585791No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGAGTATTATAATATTG18840.15119487285666822No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18660.14975033585485292No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATATAGCTCCAA18410.14774403446344278No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18320.14702176596253513No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGC18020.14461420429284297No Hit
GGCCAAAAGTGTACGGAAGAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTG17930.14389193579193532No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17920.1438116837362789No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17910.1437314316806225No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17530.14068185356567908No Hit
GCTCATTTACTGGGCGTCTACCCGGGAATCAGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT16350.13121211099822322No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16240.13032933838600275No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16230.13024908633034635No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15790.1267179958814645No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT15730.1262364835475261No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15610.1252734588796492No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15420.1237486698221775No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15220.12214362870904938No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15110.12126085609682892No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14890.119495310872388No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14790.11869279031582393No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14580.11700749714703942No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14530.1166062368687574No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14420.11572346425653693No Hit
ATCTATTATAGAGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGGGT14320.11492094369997287No Hit
GTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATATAGCTCCAACAATAAGAACTA14200.11395791903209601No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14180.1137974149207832No Hit
GCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGAATCAGGGGTC14170.1137171628651268No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14110.11323565053118836No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14010.11243312997462429No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13850.11114909708412181No Hit
CACCTGCACTGTCTCTGGGGACTCCATCAGCAGTAGTAGTTACTACTGGG13660.10962430802665012No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13500.1083402751361476No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCTTCCGTACA13500.1083402751361476No Hit
GTCCTGCCTTCTGCTGGTACCAAGCTATGTAGTTCTTATTGTTGGAGCTA13470.1080995189691784No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13460.10801926691352198No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13420.10769825869089637No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13320.10689573813433231No Hit
TCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG13300.1067352340230195No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13230.10617346963342465No Hit
ACATAGCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGCTCATT13180.10577220935514263No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13030.10456842852029655No Hit
ATACTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA12950.10392641207504531No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12840.10304363946282485No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12800.1027226312401992No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12790.1026423791845428No Hit
ATGTAGTTCTTATTGTTGGAGCTATATAAAACACTCTGGCTGGACTTGCA12650.10151885040535313No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12620.10127809423838392No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12610.10119784218272751No Hit
ATTATAGAGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGGGTCACC12570.10087683396010189No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12550.10071632984878909No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGCCTTCTGCTGGTACCAAGCTA12540.10063607779313267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)