FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246074
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51410.4125758181295814No Hit
GTTTTATATAGCTCCAACAATAAGAACTACATAGCTTGGTACCAGCAGAA44370.3560783709474718No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43010.34516409137820064No Hit
CTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGAATCAGGGGTCCCT42760.3431577899867905No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA39690.318520408900274No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39300.31539057872967413No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32810.2633069946086669No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31530.25303473148464695No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT30960.24846036431223187No Hit
GCTTAGGAGGCTGTCCTGCCTTCTGCTGGTACCAAGCTATGTAGTTCTTATTGTTGGAGCTATATAAAACACT30680.2462133067538525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30640.2458922985312269No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA27610.22157592566733594No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27340.21940912016461303No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25990.20857509265099825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25740.20656879125958813No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25310.2031179528663627No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25150.20183391997586017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24580.19725955280344507No Hit
GTTTATTACTGTCAGCAATATTATAATACTCTTCCGTACACTTTTGGCCA24160.19388896646587603No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24090.19332720207628118No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23960.1922839253527479No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23670.18995661573871214No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23630.18963560751608652No Hit
GTATATTACTGTGCCACACGTGGATACAGCTATGGTTACTTTGACTCCTGGGGCCCGGGTACCCTGGTCACCGT23600.18939485134911732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22700.1821721663400408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22680.182011662228728No Hit
TTATAATACTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22280.17880158000247176No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22170.1779188073902513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22100.17735704300065647No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21840.17527048955358993No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21650.1737457004961182No Hit
ATTATAATACTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20900.16772679632188778No Hit
GTCTCTGGGGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG20820.16708477987663653No Hit
ATATAAAACACTCTGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA20300.16291167298250345No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19860.1593805825336216No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19830.1591398263666524No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAAGAGTATTATAATATTG19330.1551272235838321No Hit
GGCCAAAAGTGTACGGAAGAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTG18910.15175663724626307No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18590.1491885714652581No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACT18370.14742302624081716No Hit
GAACTACATAGCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGC17750.14244739879012003No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17590.1411633658996175No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17270.13859530011861254No Hit
CTACATAGCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGCTCA17180.1378730316177049No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17020.1365889987272024No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16940.13594698228195115No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCTGATTCCCGGGT16920.13578647817063835No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16250.13040959044165917No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16150.12960706988509513No Hit
AGGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGAA15970.1281625328832798No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATATAGCTCCAA15900.12760076849368496No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15880.12744026438237216No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15580.12503270271267997No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT15140.12150161226379813No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15140.12150161226379813No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGC14950.11997682320632644No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCT14890.119495310872388No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14420.11572346425653693No Hit
GCTCATTTACTGGGCGTCTACCCGGGAATCAGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT14360.11524195192259849No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14320.11492094369997287No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14120.11331590258684476No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14110.11323565053118836No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13950.11195161764068585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13850.11114909708412181No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13830.110988592972809No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13830.110988592972809No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC13660.10962430802665012No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13610.10922304774836807No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13500.1083402751361476No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13500.1083402751361476No Hit
ATCTATTATAGAGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGGGT13280.1065747299117067No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13040.10464868057595295No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13030.10456842852029655No Hit
ATACTCTTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA13030.10456842852029655No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12990.10424742029767092No Hit
GTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCAGAGTGTTTTATATAGCTCCAACAATAAGAACTA12820.10288313535151201No Hit
GCTTGGTACCAGCAGAAGGCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCGTCTACCCGGGAATCAGGGGTC12720.10208061479494797No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12530.10055582573747628No Hit
GTCCTGCCTTCTGCTGGTACCAAGCTATGTAGTTCTTATTGTTGGAGCTA12510.10039532162616346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)