FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596155
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT60790.38085273673296144No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC55030.3447660158317958No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC54510.34150818686155165No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA53340.3341780716785024No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50780.31813952905576215No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC48530.30404315370374435No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG47080.29495882292133285No Hit
CTATTAGTACTTGCGAATGAGCAGCAGAGATATTCAGCCTCGTCCTCAGC41850.26219258154753144No Hit
GACCTAGGACGGTGACCTCGGTCCCAATTCCGAAGACATAACTATTAGTACTTGCGAATGAGCAGCAGAGATATT41530.2601877637196889No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41420.259498607591368No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC40840.2558648752784034No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG40810.2556769236070432No Hit
GTACTAATAGTTATGTCTTCGGAATTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA40480.25360945522208056No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT39510.2475323511814329No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36100.22616851120348586No Hit
ACCTAGGACGGTGACCTCGGTCCCAATTCCGAAGACATAACTATTAGTAC35800.22428899448988351No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTT35510.2224721283334012No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34730.21758538487803505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34400.21551791649307242No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAATTCCGAAGACATAACTATTAGTACTTGCGAATGAGCAG34270.2147034592505114No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG32340.2026119017263361No Hit
GTTATAACCCCCAACATCACTGCTGCTTCCAGTGCAGGAGATGGTGATCG30400.19045769364504073No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCAC29820.18682396133207615No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29530.18500709517559386No Hit
CTCCTGCACTGGAAGCAGCAGTGATGTTGGGGGTTATAACCGTGTCTCCTGGTACCAAAGGCACCCAGGCAAAGC29350.18387938514743243No Hit
GTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTTGGGGGTTATAACCGTGTCTCCTGGTACCAAAGGCA29310.1836287829189521No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC28230.17686252274998357No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG28130.17623601717878276No Hit
GAATATCTCTGCTGCTCATTCGCAAGTACTAATAGTTATGTCTTCGGAATTGGGACCGAGGTCACCGTCCTAGGT27960.17517095770774144No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC27020.16928180533845397No Hit
GACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCT25750.16132518458420392No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25100.15725289837139877No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24490.15343121438707394No Hit
GTACCAAAGGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGACA23860.14948422928850896No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC23100.14472278694738291No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22660.14196616243409946No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT22330.13989869404913682No Hit
GGTTATAACCGTGTCTCCTGGTACCAAAGGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGACACTAAG22180.13895893569233564No Hit
GGGTTATAACCGTGTCTCCTGGTACCAAAGGCACCCAGGCAAAGCCCCCA21820.1367035156360128No Hit
GAATTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACT21790.13651556396465256No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21640.13557580560785137No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA21500.13469869780817026No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC21180.13269387998032772No Hit
GAGATATTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG21020.13169147106640647No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG20700.12968665323856393No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20610.12912279822448322No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG20440.12805773875344187No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19860.12442400644047727No Hit
GCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCAC19830.12423605476911703No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19820.12417340421199696No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC19430.12173003248431387No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18940.11866015518543No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18870.11822160128558944No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18820.11790834849998903No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18590.1164673856862272No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18540.11615413290062683No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18460.11565292844366619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.11183124445934135No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG17440.1092625716174181No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17380.10888666827469763No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCG17310.10844811437485707No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT17090.10706980211821535No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGA17020.10663124821837479No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC16600.10399992481933146No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16480.10324811813389051No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG16200.10149390253452828No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15990.10017824083500664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)