FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596155
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC62390.3908768258721741No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT55630.3485250492590005No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC54880.3438262574749946No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48360.30297809423270294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44410.2782311241702717No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43560.272905826815065No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43200.27065040675874213No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC42870.2685829383737795No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42000.2631323399043326No Hit
CTATTAGTACTTGCGAATGAGCAGCAGAGATATTCAGCCTCGTCCTCAGC41100.25749378976352544No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG40330.25266969686527935No Hit
GTACTAATAGTTATGTCTTCGGAATTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA39760.24909861510943485No Hit
GACCTAGGACGGTGACCTCGGTCCCAATTCCGAAGACATAACTATTAGTACTTGCGAATGAGCAGCAGAGATATT37990.23800946649918084No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC36770.23036609853053117No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG36420.2281733290313284No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT35820.22441429560412363No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34120.2137637008937102No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTT33850.21207213585146806No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAATTCCGAAGACATAACTATTAGTACTTGCGAATGAGCAG33820.21188418418010782No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33480.20975406523802512No Hit
ACCTAGGACGGTGACCTCGGTCCCAATTCCGAAGACATAACTATTAGTAC31150.19515648542904668No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC29410.18425528849015288No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCAC29310.1836287829189521No Hit
GTTATAACCCCCAACATCACTGCTGCTTCCAGTGCAGGAGATGGTGATCG29150.18262637400503084No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28180.17654926996438317No Hit
CTCCTGCACTGGAAGCAGCAGTGATGTTGGGGGTTATAACCGTGTCTCCTGGTACCAAAGGCACCCAGGCAAAGC28130.17623601717878276No Hit
GTCGATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTTGGGGGTTATAACCGTGTCTCCTGGTACCAAAGGCA28050.17573481272182215No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC27200.1704095153666154No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26620.1667757830536508No Hit
GAATATCTCTGCTGCTCATTCGCAAGTACTAATAGTTATGTCTTCGGAATTGGGACCGAGGTCACCGTCCTAGGT26390.16533482023988896No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26380.16527216968276892No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26200.16414445965460747No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24970.15643844112883773No Hit
GGGTTATAACCGTGTCTCCTGGTACCAAAGGCACCCAGGCAAAGCCCCCA24790.1553107311006763No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC24620.15424567162963498No Hit
GACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCT23110.144785437504503No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22670.14202881299121953No Hit
GGTTATAACCGTGTCTCCTGGTACCAAAGGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGACACTAAG22550.14127700630577858No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC22550.14127700630577858No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21760.13632761229329232No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC21400.13407219223696948No Hit
GTACCAAAGGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGACA20960.13131556772368597No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20850.13062641159536512No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20530.12862159376752258No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA20420.1279324376392017No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC20100.12592761981135916No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT19870.12448665699759737No Hit
GAGATATTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG19300.12091557524175284No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG19270.1207276235703926No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19270.1207276235703926No Hit
GCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCAC19130.11985051577071149No Hit
GAATTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACT19030.1192240101995107No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19030.1192240101995107No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18640.11678063847182761No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18540.11615413290062683No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18150.11371076117294374No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17930.112332448916302No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGA17880.11201919613070159No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG17070.10694450100397518No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCG17030.10669389877549487No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG17010.10656859766125469No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16920.10600474264717398No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16420.10287221479117004No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG16120.10099269807756765No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16050.1005541441777271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG100.0085648615136.1207370
CCTAACG701.0913936E-1148.166752
CTAACGC752.1827873E-1144.9514123
GGTATCA23350.044.3438071
GTGGTAT6400.043.2130971
TGGTATC6900.039.091862
GTATCAA59950.037.63731
AACCGTA400.002305411535.3468651
TATCAAC68550.032.6592372
ATCAACG69750.031.9976733
TCAACGC70550.031.5880364
CAACGCA72050.031.0220535
AACGCAG72450.030.8507796
TACGTTG500.005273529329.85216366
TAACGCC1251.6370905E-1029.6688614
ACGCAGA76500.029.2175037
CGCAGAG76550.029.198428
AGACCGT1052.9191142E-828.8964316
AGAGTAC77950.028.45687311
GCAGAGT78800.028.279149
Produced by FastQC (version 0.11.5)