FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331559
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70120.5266007739799738No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT61890.46479352398203905No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA59480.4466944386241991No Hit
GCCTTGGGCTGACCAAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTG53300.400282676171315No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG49860.37444829707132765No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48950.3676142026001101No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG47700.35822671019459146No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC44500.33419472963646374No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39030.2931150628699141No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT38980.2927395631736934No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC38810.2914628642065428No Hit
GATTATCACTGCAGCTCATTTACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTTGGT36340.27291317921323804No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTT35190.26427668620016087No Hit
GACCAAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTGCAGTGATAAT34310.25766789154667574No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAATTCTGTCTCCTGGTACCAACAACACCCAGACAAAGC33490.2515096965286555No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32200.24182180436616027No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAATTCTGTCTCCTGGTACCAACAACA31970.24009450576354482No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31560.2370154082545347No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31470.2363395088013374No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAAGTGCTG30460.22875441493767829No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30020.22545001761093575No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG30020.22545001761093575No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29290.21996772204611287No Hit
GTGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG26420.19841403948304207No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26340.19781323996908884No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25890.19443374270310215No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT25250.18962734659147662No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24940.18729924847490798No Hit
GAATTATAACTACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT24930.18722414853566383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24710.18557194987229256No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA23930.17971415461124893No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23550.17686035691997123No Hit
CCCCTGAGGGGCGATTACTGACCTCATAAATCAAGAGTTTGGGGGCTTTGTCTGGGTGTTGTTGGTACCAGGAGA23160.17393145928944945No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT22570.16950056287404464No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21670.1627415683420712No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21450.16108936967869994No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC21360.1604134702255026No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20880.15680867314178343No Hit
GTAATCGCCCCTCAGGGGTTTCTATTCGCTTCTCTGGCTCCAAGTCTGGC20840.15650827338480683No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20640.15500627459992386No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20440.15350427581504086No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20260.1521524769086462No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG20150.15132637757696055No Hit
CCTCAGGGGTTTCTATTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG19910.14952397903510098No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19580.14704568104004404No Hit
GCACTTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTTGGTCAGCCC19350.14531838243742862No Hit
TTATAATTCTGTCTCCTGGTACCAACAACACCCAGACAAAGCCCCCAAACTCTTGATTTATGAGGTCAGTAATCG19320.14509308261969617No Hit
CATAAGTGCTGCTGCTTGTAAATGAGCTGCAGTGATAATCAGCCTCGTCC19120.14359108383481317No Hit
GTTATAATTCTGTCTCCTGGTACCAACAACACCCAGACAAAGCCCCCAAA18990.14261478462463925No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18770.14096258596126796No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCAGCAGCACTTATGT18770.14096258596126796No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18390.1381087882699903No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA18210.13675698936359562No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC18200.13668188942435147No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18100.13593089003190997No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG17940.13472929100400358No Hit
CACCCAGACAAAGCCCCCAAACTCTTGATTTATGAGGTCAGTAATCGCCCCTCAGGGGTTTCTATTCGCTTCT17750.13330239215836476No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17740.1332272922191206No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG17430.13089919410255196No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17280.12977269501388974No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17060.12812049635051845No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16970.1274445968973211No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16910.12699399726185623No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCA16900.12691889732261208No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16640.12496629890226418No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC16450.12353940005662535No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16220.12181210145400993No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG16080.12076070230459184No Hit
CTCTTGATTTATGAGGTCAGTAATCGCCCCTCAGGGGTTTCTATTCGCTT15970.11993460297290619No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15950.1197844030944179No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA15820.11880810388424395No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15800.11865790400575565No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15340.11520330680052479No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC15080.11325070838017692No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA15080.11325070838017692No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15070.11317560844093276No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGAAT14950.11227440917000299No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG14900.11189890947378223No Hit
CAGCAGCACTTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTTGGTCAGCCCAAGGCCAACCCCACTGTCA14880.11174870959529393No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14800.11114791008134074No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14480.10874471202552798No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT14290.10731781317988914No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT14280.107242713240645No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG14200.1066419137266918No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG14080.105740714455762No Hit
GGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAAGTCAC14010.10521501488105296No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA13720.10303711664297263No Hit
GTCTGGGTGTTGTTGGTACCAGGAGACAGAATTATAACTACCAATGTCAC13710.10296201670372848No Hit
GTGCTGCTGCTTGTAAATGAGCTGCAGTGATAATCAGCCTCGTCCTCAGC13630.10236121718977528No Hit
AATCAAGAGTTTGGGGGCTTTGTCTGGGTGTTGTTGGTACCAGGAGACAGAATTATAACTACCAATGTCACTG13320.10003311907320668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4400.053.8594370
CACCGTA200.00650764653.384657
TATTCGT400.00792810150.78174670
TCGTATG3850.046.59448640
TCTCGTA3650.046.1361238
CGTATGC3950.045.4756241
TATACAC4200.044.4664843
ATGCCGT4100.044.0255244
ATCTCCG4150.043.3543810
GGTATCA11800.042.883371
ATCTCGT4000.042.04167637
GCCGTCT4350.040.8305946
CGAGACT4500.040.75053423
ACGAGAC4500.040.7459422
CACGAGA4450.040.4361721
TAGGCAT4400.040.23132329
CCCACGA4550.039.54597519
CTTATAC4900.038.8129461
ATACACA4900.038.8071144
AGACTAG4700.038.3155325
Produced by FastQC (version 0.11.5)