FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331559
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61660.46306622537942366No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT56880.4271684544207204No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC54800.4115476670579373No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA53810.4041127730727666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50080.37610049573469895No Hit
GCCTTGGGCTGACCAAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTG49490.37166959931929416No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG47720.35837691007307976No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC45110.3387758259303568No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG44020.33058993255274455No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40500.3041547539388041No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT38500.28913476608997424No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35410.26592888486353217No Hit
GATTATCACTGCAGCTCATTTACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTTGGT35080.2634505868684752No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33290.2500076977437725No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAATTCTGTCTCCTGGTACCAACAACACCCAGACAAAGC32940.24737919987022733No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTT32720.24572700120685603No Hit
GACCAAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTAAATGAGCTGCAGTGATAAT32670.24535150151063526No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAATTCTGTCTCCTGGTACCAACAACA32490.2439997026042406No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32310.2426479036978459No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC32170.24159650454842782No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAAGTGCTG31330.23528810965191932No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31230.2345371102594778No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28950.2174143241118118No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG28610.21486092617751074No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26010.19533494197403195No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT25140.18880124725979097No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG25120.18865104738130267No Hit
GTGATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG24940.18729924847490798No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24560.1844454507836303No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23810.17881295534031913No Hit
GAATTATAACTACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT23680.17783665613014518No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA23640.17753625637316858No Hit
CCCCTGAGGGGCGATTACTGACCTCATAAATCAAGAGTTTGGGGGCTTTGTCTGGGTGTTGTTGGTACCAGGAGA23190.17415675910718187No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23110.17355595959322867No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22200.16672186512201112No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21100.15846087180515472No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20810.15628297356707438No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20520.15410507532899406No Hit
GTAATCGCCCCTCAGGGGTTTCTATTCGCTTCTCTGGCTCCAAGTCTGGC20480.15380467557201746No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19860.1491484793388802No Hit
TTATAATTCTGTCTCCTGGTACCAACAACACCCAGACAAAGCCCCCAAACTCTTGATTTATGAGGTCAGTAATCG19470.14621958170835841No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG19380.14554368225516107No Hit
CCTCAGGGGTTTCTATTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG19320.14509308261969617No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19300.14494288274120784No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19140.14374128371330147No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19100.1434408839563249No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18390.1381087882699903No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18290.1373577888775488No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCAGCAGCACTTATGT18200.13668188942435147No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC18020.13533009051795677No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17960.13487949088249188No Hit
GCACTTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTTGGTCAGCCC17950.13480439094324773No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17440.1309742940417961No Hit
CATAAGTGCTGCTGCTTGTAAATGAGCTGCAGTGATAATCAGCCTCGTCC17440.1309742940417961No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17320.13007309477086632No Hit
GTTATAATTCTGTCTCCTGGTACCAACAACACCCAGACAAAGCCCCCAAA17290.1298477949531339No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG17230.12939719531766897No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17160.12887149574295995No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17120.12857109598598335No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17100.12842089610749505No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG17050.1280453964112743No Hit
CACCCAGACAAAGCCCCCAAACTCTTGATTTATGAGGTCAGTAATCGCCCCTCAGGGGTTTCTATTCGCTTCT16740.12571729829470568No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16610.12474099908453173No Hit
CTCTTGATTTATGAGGTCAGTAATCGCCCCTCAGGGGTTTCTATTCGCTT16510.12398999969209024No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT16420.1233141002388929No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16400.12316390036040462No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA15780.11850770412726735No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC15530.11663020564616362No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG15480.11625470594994289No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15210.11422700759035087No Hit
GTGCTGCTGCTTGTAAATGAGCTGCAGTGATAATCAGCCTCGTCCTCAGC15200.11415190765110672No Hit
CAGCAGCACTTATGTCTTCGGAACTGGGACCAAAGTCACCGTCCTTGGTCAGCCCAAGGCCAACCCCACTGTCA15010.11272500880546787No Hit
GCTCCAGGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCA15010.11272500880546787No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG14950.11227440917000299No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14770.1109226102636083No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT14710.1104720106281434No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14650.11002141099267851No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14580.10949571141796947No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC14540.10919531166099286No Hit
GTCTGGGTGTTGTTGGTACCAGGAGACAGAATTATAACTACCAATGTCAC14410.10821901245081894No Hit
GGCTGAGGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCAGCAGCA14380.10799371263308648No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA14260.10709251336215668No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC14190.10656681378744765No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG14180.1064917138482035No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA14140.10619131409122691No Hit
GGACGAGGCTGATTATCACTGCAGCTCATTTACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAAGTCAC14110.10596601427349446No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13910.10446401548861146No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC13500.10138491797960136No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13490.10130981804035721No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG13420.10078411846564816No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC13350.10025841889093912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)