FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636429
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC77500.47359219373403916No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGCCTGCATATGAGCAG70910.43332158009910604No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT65290.3989785074696183No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61140.37361840935353746No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGCCTGCATATGAGCAGCAGTAATAAT55980.34208633555137435No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC55670.3401919667764382No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG54800.3348755124725851No Hit
GATTATTACTGCTGCTCATATGCAGGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGT54660.3340199910903559No Hit
GTATACGACGGGGTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCT54540.3332866870484451No Hit
GTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG51300.31348747791685433No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT49780.30419896005265123No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG45510.2781055578946596No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44120.26961145274252657No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC42930.26233952099357805No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG42640.26056736955896037No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT42570.2601396088678458No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42460.25946741349609426No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41160.25152328637539423No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT40760.249078939569025No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39630.2421736598410319No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC39090.23887379165243344No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC38850.2374071835686119No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38270.2338628806993765No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC37990.23215183793491803No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37220.22744647033265727No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA36610.22371884145294418No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG36340.22206890735864496No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT35920.21950234321195727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35600.21754686576686186No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35230.2152858449709703No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35110.21455254092905954No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34100.20838056524297724No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33560.20508069705437879No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA33470.20453071902294567No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC33280.20336965428992032No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32570.1990309387086149No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31950.1952422011587426No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31620.19322561504348798No Hit
GTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC30080.1838148798389664No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTCAGTAAG29700.18149275037291565No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28140.17195979782807566No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGAC27990.17104316777568718No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27930.1706765157547318No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT27770.1696987770321841No Hit
GGGTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC27130.16578782214199334No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC27030.165176735440401No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26960.1647489747492864No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG26940.16462675740896793No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC26880.16426010538801256No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26760.16352680134610179No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA26670.1629768233146687No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC25660.15680484762858637No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC25280.15448271816253561No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG24630.15051065460218563No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24510.14977735056027486No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24490.14965513321995638No Hit
GCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAG24100.14727189508374636No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG23920.14617193902088021No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23910.14611083035072098No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23380.14287207083228176No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC23350.14268874482180405No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23230.14195544077989328No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTCA23200.1417721147694156No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22560.13786115987922481No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGC22530.13767783386874713No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22210.13572235642365174No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22010.13450018302046712No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG21970.1342557483398302No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG21920.13395020498903407No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT21800.1332169009471233No Hit
CACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA21750.13291135759632713No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21230.12973370674804713No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT21000.1283282073343848No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20920.12783933797311098No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC20850.12741157728199637No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCC20580.12576164318769711No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC20470.12508944781594558No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20380.1245394697845125No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC20360.12441725244419403No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT20260.12380616574260173No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGA20070.12264510100957633No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19910.12166736228702865No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19790.12093405824511788No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTC19790.12093405824511788No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19770.1208118409047994No Hit
GGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGG19700.1203840802136848No Hit
GGCAAAGCCCCCAAACTCCTGATTTATGATGTCAGTAAGCGGCCCTCAGG19650.12007853686288865No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC19050.1164120166533348No Hit
CCTGAGGGCCGCTTACTGACATCATAAATCAGGAGTTTGGGGGCTTTGCC18960.11586203862190173No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18910.11555649527110556No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG18850.11518984325015018No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC18770.11470097388887633No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18710.11433432186792095No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18690.11421210452760247No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18650.11396766984696556No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18520.11317325713489557No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTA18500.11305103979457709No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18210.1112788883599594No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC18180.1110955623494817No Hit
GCTCATATGCAGGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG17980.10987338894629708No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17680.10804012884152016No Hit
GACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCT17560.10730682479960939No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17260.10547356469483248No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC17070.1043124999618071No Hit
GTTGGTACCAGGAGACATAGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC16980.103762521930374No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA16740.10229591384655246No Hit
CTCCTGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTT16600.10144039246432324No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16590.101379283794164No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG16530.10101263177320861No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAA16520.10095152310304939No Hit
GTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC16490.10076819709257169No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT16400.10021821906113862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)