FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636429
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC66820.40832813400398066No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG65430.3998340288518475No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGCCTGCATATGAGCAG63560.38840670753207135No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT59010.3606022626096213No Hit
GATTATTACTGCTGCTCATATGCAGGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGT52340.31984277961341434No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG52320.31972056227309587No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGCCTGCATATGAGCAGCAGTAATAAT50700.30982095770730045No Hit
GTATACGACGGGGTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCT50070.3059711114872689No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48980.29931026643991276No Hit
GTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG48480.29625483293195126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43580.2663115845539281No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG43280.26447832444915115No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG43140.26362280306692193No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42100.2572675013703619No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG41830.2556175672760627No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG40250.24596239739090422No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT40200.24565685404010806No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40040.24467911531756034No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC40030.24461800664740113No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39360.24052372574673267No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC39310.24021818239593654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38400.2346572934114465No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT37390.22848531772536418No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36290.22176336400784882No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35880.21925790853132032No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35380.21620247502335876No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA34280.2094805213058434No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34210.20905276061472877No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC34210.20905276061472877No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34200.20899165194456953No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG34010.20783058721154413No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33870.2069750658293149No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33040.20190304620609878No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32320.19750322195463416No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31940.19518109248858337No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA31570.19292007169269182No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG30660.18735918270820184No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT30090.18387598850912568No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28780.17587075271826644No Hit
GTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC28710.1754429920271518No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGAC28580.17464857931508182No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTCAGTAAG28000.17110427644584641No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26870.16419899671785332No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26810.16383234469689792No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25970.15869921640352255No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25920.1583936730527264No Hit
GGGTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC25690.1569881736390641No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC25620.15656041294794948No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT25530.1560104349165164No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG25220.15411606614158024No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC25040.15301611007871407No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA24800.15154950199489253No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24770.15136617598441485No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24520.14983845923043407No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC23900.14604972168056177No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT23800.14543863497896947No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23700.14482754827737715No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC23690.1447664396072179No Hit
GCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAG23320.14250541881132636No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23180.14164989742909714No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC23180.14164989742909714No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG22940.1401832893452756No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTCA22550.1378000512090656No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22030.13462240036078557No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG21630.13217805355441636No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC21130.1291226200464548No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT20450.1249672304756271No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20310.12411170909339787No Hit
GTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG19980.12209512297814326No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT19930.12178957962734711No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19900.12160625361686943No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC19890.12154514494671018No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG19840.12123960159591404No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTC19770.1208118409047994No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19570.11958966750161479No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGTATACGACGGGGTACGTGC19550.11946745016129634No Hit
CACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA19440.1187952547895448No Hit
GGCACGTACCCCGTCGTATACGGCGGAGGGACCAAGCTGACCGTCCTAGG19350.11824527675811172No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19270.11775640739683788No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT19190.11726753803556401No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18780.11476208255903557No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATC18630.11384545250664709No Hit
GGCAAAGCCCCCAAACTCCTGATTTATGATGTCAGTAAGCGGCCCTCAGG18480.11292882245425863No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC18390.11237884442282557No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18370.11225662708250708No Hit
CCTGAGGGCCGCTTACTGACATCATAAATCAGGAGTTTGGGGGCTTTGCC18230.11140110570027786No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCC18060.11036225830757093No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC17830.10895675889390863No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17610.10761236815040555No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGA17540.10718460745929093No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC17490.10687906410849478No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGATGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG17460.10669573809801709No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17310.10577910804562861No Hit
GTGCTGTTGGTACCAGGAGACATAGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA17280.10559578203515094No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17190.10504580400371784No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17130.10467915198276245No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC17010.10394584794085171No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16720.10217369650623401No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16570.10125706645384555No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAA16540.10107374044336784No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTA16430.1004015450716163No Hit
GTTGGTACCAGGAGACATAGTTATAACCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTC16390.1001571103909794No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16370.1000348930506609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG200.007769084551.046032
GTGGTAT4300.045.151861
GTATCAA33050.038.235931
CCGGATG800.00188511736.82416570
GGTATCA17100.035.456281
TATCAAC36800.034.1231582
ATCAACG37800.033.032293
TCAACGC38100.032.7641834
CAACGCA38550.032.3807335
AACGCAG39000.031.9198956
ACGCAGA41000.030.279877
AATGGTA4450.029.79033570
CGCAGAG42000.029.6399028
AGAGTAC42550.029.4920811
GCAGAGT43850.028.6988079
AACTATT1100.00895694926.7812170
GATTGAC1405.4387783E-1026.7629431
CAGAGTA47650.026.48150810
GCTTATA659.3293516E-426.201481
TGGTATC7550.026.1427752
Produced by FastQC (version 0.11.5)