FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821151
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC78280.42983805296760125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76090.41781269098498697No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC62980.3458252500753644No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61280.33649049419844923No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60890.3343489913796275No Hit
GTCCTGGTCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG51710.2839413096442854No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47600.2613731645536257No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC45630.25055582980214164No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT44320.24336257674404815No Hit
GGACAGAAGGTCACCATCTCCTGCTCCGGAAGCAGCTCCAACATTGGGAGTAATTATGTATCCTGGTACCAGCTT43420.23842064716215186No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC42710.23452201382532256No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGGCTGTTATCCCATGTTCCGCAGTAATAATCGG42680.23435728283925936No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41590.22837205701229604No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40900.22458324433284227No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG39720.21810382554768934No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37210.2043213330470675No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36510.20047761003892592No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36270.19915976215042025No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35680.1959200527578438No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG35180.19317453632345696No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG34480.18933081331531543No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34050.18696966918174274No Hit
GATTATTACTGCGGAACATGGGATAACAGCCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG33990.18664020720961633No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33860.18592637293667577No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33180.18219247058590968No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCCGG32890.18060007105396533No Hit
GGGTCCTGGTCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC32420.1780192856056417No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31790.17455993489831428No Hit
GTCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGAC30960.1700023776172322No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30850.16939836400166708No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAA30710.16862961940003876No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG30500.1674765024975963No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT30380.16681757855334345No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG30310.1664332062525293No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30270.16621356493777836No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29970.16456625507714628No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG29630.1626993039017632No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC29610.16258948324438777No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28200.1548471268994169No Hit
GGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCATGTGGTATT27770.15248598276584424No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGTCCCAGT27730.1522663414510933No Hit
GGATAACAGCCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT27520.1511132245486508No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26880.14759896351263568No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26850.14743423252657248No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26460.14529272970775076No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26200.14386506116186962No Hit
GTTTGGGGGCTGTTCCTGGGAGAAGCTGGTACCAGGATACATAATTACTC25410.1395271451955384No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25380.1393624142094752No Hit
GCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGT25020.13738564237671672No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC24860.13650707711771293No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24770.13601288415952328No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24760.13595797383083555No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCC24640.13529904988658273No Hit
CTCCTGCTCCGGAAGCAGCTCCAACATTGGGAGTAATTATGTATCCTGGTACCAGCTTCTCCCAGGAACAGCCCC23540.12925891373093168No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23230.1275566935416119No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22750.12492099776460053No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC22610.12415225316297221No Hit
GCTTGGTCCCTCCGCCGAATACCACATGGCTGTTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGT22520.12365806020478258No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC22450.12327368790396842No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC22300.12245003297365238No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22010.12085763344170802No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC21870.12008888884007972No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG21840.1199241578540165No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT21460.11783756536388251No Hit
CCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGTCCCAG21050.11558624188768532No Hit
GCACAGGGTCCTGGTCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT21000.11531169024424663No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20650.11338982874017586No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAACAGCCATGTGGTATTCGGCGGAGGGAC20470.11240144282379659No Hit
TCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGG20360.1117974292082315No Hit
GCCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC20100.11036976066235034No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA20090.1103148503336626No Hit
GTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTC20000.10982065737547299No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19880.10916173343122014No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19510.10713005126977389No Hit
CCACATGGCTGTTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCA19310.10603184469601917No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19150.10515327943701538No Hit
GCTCCAACATTGGGAGTAATTATGTATCCTGGTACCAGCTTCTCCCAGGA18930.10394525220588519No Hit
CAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACC18610.10218812168787761No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18600.10213321135918987No Hit
GTTCCTGGGAGAAGCTGGTACCAGGATACATAATTACTCCCAATGTTGGA18460.10136446675756156No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18370.10087027379937193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG452.1252617E-645.124912
CTAACGC504.394731E-640.6012653
GGTATCA20150.039.4887351
GTATCAA43850.033.43481
TGGTATC7350.029.9297892
TAACGCC704.401378E-528.9993114
TATCAAC51450.028.2852942
ATCAACG51600.027.9986153
TCAACGC52250.027.6487884
CTAGTAC500.00850740327.067513
ACGAAAC500.00850740327.067513
CAACGCA53550.027.0407545
AACGCAG53850.026.9529386
CGCAGAG56700.025.6585318
AGAGTAC57300.025.3891611
ACGCAGA57250.025.3522387
CAGAGTA57700.025.09588210
AATGGTA4950.024.9077570
GCAGAGT58550.024.9055889
GTGGCCG9250.023.6960270
Produced by FastQC (version 0.11.5)