FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364636
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA42460.31114524312710495No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG38590.28278603231924115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34910.2558191341866989No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCGAGAGTGCTG34860.25545273611424585No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34200.25061628155786597No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC34200.25061628155786597No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT34060.24959036695499753No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31940.23405508868298947No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC31120.2280461602947599No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC29580.2167610996632069No Hit
CCCCGAGAGTGCTGCTGCCTGCATATGAGTTGCAGTAATAATCAGCCTCG29250.21434287238501695No Hit
CTCTCGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC28650.2099460955155807No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27710.20305781175346393No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCATAT27390.2007128640897646No Hit
GATTATTACTGCAACTCATATGCAGGCAGCAGCACTCTCGGGGTGTTCGG26190.1919193103508921No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25750.18869500731330552No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC25660.18803549078289009No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25300.18539742466122833No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24920.18261279931058538No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24130.17682370976582767No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA23930.17535811747601557No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23450.17184069598046658No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23380.17132773867903237No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23370.17125445906454173No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23350.17110789983556055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23160.16971558716023907No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22680.1661981656646901No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22270.1631937014705753No Hit
GTGTATTACTGTGCAAGGCCATATTATTATGATAGTAGTGGTTATTCACCCCTTGACTACTGGGGCCAGGGAACC21460.15725805269683638No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21350.15645197693743973No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20700.15168880199555046No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20640.15124912430860685No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20560.15066288739268202No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20220.14817138050000148No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20050.1469256270536612No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19960.14626611052324576No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCGTGATTTATGATGTCAGTAAT19780.1449470774624149No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19010.13930454714663837No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18930.13871831023071354No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18850.13813207331478872No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18730.13725271794090144No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18390.13476121104822092No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18070.1324162633845216No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17840.1307308322512377No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17340.1270668515267075No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17280.12662717383976385No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16590.12157088043991218No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16400.1201785677645907No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG16200.11871297547477863No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15990.11717410357047593No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCGTGATTTATGATGTC15910.1165878666545511No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15640.1146093170633048No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15480.11343684323145513No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15220.11153157325469942No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15190.1113117344112276No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC15120.11079877710979337No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG14360.10522952640850747No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTACTGGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG13880.10171210491295847No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13790.10105258838254304No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13670.10017323300865578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT252.6088624E-455.0210139
TCGCGTA306.464365E-445.77763738
GGTATCA10750.044.755221
TTAACGG704.838512E-1043.96966635
CGGTACC758.1490725E-1041.6858945
CACGGAT700.001304032339.69190270
CGAGACT1850.038.47146223
ATCGCGT451.0396949E-438.09145737
GGATGGG10250.037.94932670
TCTGGAG14150.035.8349170
CACGAGA1800.035.76783421
ACGAGAC2000.035.58349622
GTATCAA24600.035.1211621
GCGTATG400.002611816634.45861440
CTAGACA400.002836260233.884074
CGAGCCC1900.033.8828315
TGGTATC2900.032.713262
TAACGGC959.491487E-932.43108736
CGTATGC652.308921E-531.87209341
TTATACA2350.031.7189372
Produced by FastQC (version 0.11.5)