FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321130
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39070.2957316842400067No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33520.2537221923656264No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCG27070.20490035045756283No Hit
ATCTGAAGTCGCCTCAAACCTTCGGCCAAGGGACCAAAGTGGAAATCAAA26440.20013170543398454No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25200.190745800943132No Hit
GTCTACCACAGCGGAAGCACCAACTTCAACCCTTCCCTCAAGAGTCGAGT25120.19014025871791573No Hit
CATCTATGGTGCATCCACGAGGGCCACTGGCACGCCAGACAGGTTCAGTG23440.1774238719883736No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23230.17583432364718082No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22990.17401769697153194No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22530.17053582917653826No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22530.17053582917653826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22090.16720534693784866No Hit
GTCTCTGGTGGCTCCGTCGGTGCTCATTACTGGAGCTGGATCCGGCAGTC22060.16697826860339254No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20500.15517019521167486No Hit
GTACTACAGGAGTCGGGCCCAGGGGTAGTGAAGGCCTCGGAGACCCTGTCCCTCACCTGCTCTGTCTCTGGTGG20380.15426188187385043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20270.15342926131417803No Hit
GGATACCCTGGCCCCAGACGTCAAAGTAGTTTTCGTCCCAGCCACTGTGA19880.1504772429662486No Hit
GTGTGGAGCCTGACGATTTCGGTGTATTTTACTGTCAGCACTATCTGAAGTCGCCTCAAACCTTCGGCCAAGGGA19730.14934185129396804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19450.14722245350571103No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19250.14570859794267027No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCTGCCCCCAGATGGGTC19200.14533013405191011No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18670.14131841680985216No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17950.13586853678290556No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17640.1335220606601924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17620.13337067510388834No Hit
GTCTCCAGGCACCCTGTCTTCGTCTCCAGGGGAAAGAGCCACTCTCTCGTGCAGGGCCAGTCAGAGTGTTGAGAA17360.1314026628719354No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17300.13094850620302317No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17250.130570042312263No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17170.12996450008704669No Hit
ACTTCAGATAGTGCTGACAGTAAAATACACCGAAATCGTCAGGCTCCACACTGCTGATGGTGAGAATGAAGT17090.1293589578618304No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17060.1291318795273743No Hit
TCTATGGACACGTCCAAGAACCAGTTCACCCTGAGCCTGGGCTCTGTGAC16790.1270881745172693No Hit
ATAGTAAGTGGCCGTGTCTGCGGCGGTCACAGAGCCCAGGCTCAGGGTGA16250.12300076449705934No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16040.12141121615586656No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAAGGTTTGAGGCGACTT15960.12080567393065028No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15910.1204272100398901No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15730.11906474003315344No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15540.11762657724826474No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15510.11739949891380863No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15320.11596133612891994No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAAGGTT15250.11543148668185568No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15120.1144474805658792No Hit
CTCCAGGCACCCTGTCTTCGTCTCCAGGGGAAAGAGCCACTCTCTCGTGC15110.11437178778772718No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15060.113993323896967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14910.11285793222468644No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14840.11232808277762218No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14720.11141976943979774No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGCGACCAGGATACCCTGGCCCCAGACGTCAAAGTAGTTTTCGT14710.11134407666164572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14160.10718097386328371No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13890.10513726885317873No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13820.10460741940611447No Hit
CTCCAGAAGCCTGGCCAGTCTCCCCGTCTCCTCATCTATGGTGCATCCAC13600.10294217828676966No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGCGACCAGGATACCCTGG13540.10248802161785743No Hit
GACTTCATTCTCACCATCAGCAGTGTGGAGCCTGACGATTTCGGTGTATTTTACTGTCAGCACTATCTGAAGTCG13520.10233663606155337No Hit
CCATAGATGAGGAGACGGGGAGACTGGCCAGGCTTCTGGAGATACCAGGC13510.10226094328340132No Hit
CTCTCGTGCAGGGCCAGTCAGAGTGTTGAGAACAACTTAGCCTGGTATCTCCAGAAGCCTGGCCAGTCTCCCCGT13460.10188247939264115No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13450.10180678661448911No Hit
CCCTGAGCCTGGGCTCTGTGACCGCCGCAGACACGGCCACTTACTATTGTGCGAAACATGAGCATCACAGTGG13390.10135262994557688No Hit
GTCCCTCACCTGCTCTGTCTCTGGTGGCTCCGTCGGTGCTCATTACTGGA13360.10112555161112077No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13310.1007470877203606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)