FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321130
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37840.28642147252730615No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28800.21799520107786516No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25970.19657414486083882No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAATCG25020.18938333093639537No Hit
GTCTACCACAGCGGAAGCACCAACTTCAACCCTTCCCTCAAGAGTCGAGT24850.18809655370781073No Hit
ATCTGAAGTCGCCTCAAACCTTCGGCCAAGGGACCAAAGTGGAAATCAAA24540.18575007758509762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23490.17780233587913377No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22070.1670539613815446No Hit
GTGTGGAGCCTGACGATTTCGGTGTATTTTACTGTCAGCACTATCTGAAGTCGCCTCAAACCTTCGGCCAAGGGA21910.165842876931112No Hit
GTCTCTGGTGGCTCCGTCGGTGCTCATTACTGGAGCTGGATCCGGCAGTC21410.1620582380235102No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCTGCCCCCAGATGGGTC21160.16016591856970924No Hit
CATCTATGGTGCATCCACGAGGGCCACTGGCACGCCAGACAGGTTCAGTG20580.15577573743689113No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20470.15494311687721873No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20410.15448896020830652No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20150.15252094797635357No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20110.15221817686374542No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20090.15206679130744136No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19470.1473738390620151No Hit
GTACTACAGGAGTCGGGCCCAGGGGTAGTGAAGGCCTCGGAGACCCTGTCCCTCACCTGCTCTGTCTCTGGTGG18850.14268088681658883No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18680.14139410958800422No Hit
GGATACCCTGGCCCCAGACGTCAAAGTAGTTTTCGTCCCAGCCACTGTGA18560.14048579625017976No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17810.134808837888777No Hit
ACTTCAGATAGTGCTGACAGTAAAATACACCGAAATCGTCAGGCTCCACACTGCTGATGGTGAGAATGAAGT17040.12898049397107023No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16390.12406046339118784No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16350.1237576922785797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16250.12300076449705934No Hit
TCTATGGACACGTCCAAGAACCAGTTCACCCTGAGCCTGGGCTCTGTGAC16210.1226979933844512No Hit
GTCTCCAGGCACCCTGTCTTCGTCTCCAGGGGAAAGAGCCACTCTCTCGTGCAGGGCCAGTCAGAGTGTTGAGAA16160.122319529493691No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16110.12194106560293083No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16070.12163829449032268No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16060.12156260171217065No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15980.12095705948695434No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAAGGTT15610.118156426695329No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15600.11808073391717695No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15530.1175508844701127No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15330.11603702890707199No Hit
ATAGTAAGTGGCCGTGTCTGCGGCGGTCACAGAGCCCAGGCTCAGGGTGA15320.11596133612891994No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15170.11482594445663939No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15090.11422040223142309No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14770.11179823333055794No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGCGACCAGGATACCCTGGCCCCAGACGTCAAAGTAGTTTTCGT14700.11126838388349368No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14670.11104130554903757No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14660.11096561277088553No Hit
CTCCAGGCACCCTGTCTTCGTCTCCAGGGGAAAGAGCCACTCTCTCGTGC14540.11005729943306108No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14260.10793790164480407No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14250.10786220886665203No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14190.10740805219773981No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14010.10604558219100317No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAAGGTTTGAGGCGACTT13970.10574281107839502No Hit
CCCTGAGCCTGGGCTCTGTGACCGCCGCAGACACGGCCACTTACTATTGTGCGAAACATGAGCATCACAGTGG13970.10574281107839502No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13880.10506157607502668No Hit
GACTTCATTCTCACCATCAGCAGTGTGGAGCCTGACGATTTCGGTGTATTTTACTGTCAGCACTATCTGAAGTCG13760.10415326273720224No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13710.10377479884644207No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGCGACGGCGACCAGGATACCCTGG13540.10248802161785743No Hit
GTGGTAGACATAGCCGATCCACTCCGGCGACTTCCCTGGGGACTGCCGGA13540.10248802161785743No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13330.10089847327666467No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13250.10029293105144838No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13230.10014154549514431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAT350.00560501455.4354770
GGTATCA13500.044.704381
ACCGACG6450.038.10293670
GTATCAA33900.035.4029581
GTGGTAT3350.034.802091
TATCAAC36900.032.3277932
ATCAACG38500.030.8835643
TCAACGC39000.030.5708524
CAACGCA39750.030.1652835
AACGCAG40300.029.8386086
TAACCGT605.0154043E-429.1360253
CGCAGAG42550.028.0181668
AACCGTG755.5918426E-527.99761854
TCGAAGG955.084932E-627.91130868
AGAGTAC43200.027.59659811
ACGCTTT550.00780540827.54882268
ACGCAGA43700.027.4386737
GCAGAGT44200.026.972249
CAGAGTA46500.025.71180210
GATATAC700.001382188524.4930651
Produced by FastQC (version 0.11.5)