FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076855
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT48350.4489926684651137No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGTTAAGTAGTTGTTATTGTTGGAGGTGTATAAAATATGG41470.3851029154342971No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT37650.3496292444200937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36540.33932144996308694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30260.2810034777198416No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27360.2540732039132474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26970.25045154640132605No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA25690.23856508072117416No Hit
ATTTTATACACCTCCAACAATAACAACTACTTAACTTGGTACCAACAGAA24790.23020740953981733No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24710.22946450543480781No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21690.2014198754706994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21110.19603382070938055No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20060.18628320433113094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19640.18238295777983107No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT19430.18043283450418116No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19400.1801542454648026No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA19110.17746121808414317No Hit
TTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18910.17560395782161944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18610.17281806742783384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18470.17151798524406722No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18460.17142512223094103No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18250.16947499895529108No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17490.16241740995770088No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.15127384838255847No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16200.1504380812644228No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15950.14811650593626813No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15610.14495916348997775No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA15560.14449484842434682No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14920.13855161558427087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14910.13845875257114468No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14490.13455850601984481No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT14090.13084398549479737No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGC13920.12926531427165217No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT13900.1290795882453998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13630.12657228689099276No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13450.12490075265472139No Hit
GTATAAAATATGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAG13430.124715026628469No Hit
GTGTATTACTGTGCAAGAGGTTTACAGCAGAACAGCCATAACATGGACGT13400.12443643758909045No Hit
GTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGTTA13340.12387925951033332No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13180.12239345130031433No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13180.12239345130031433No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13140.12202199924780958No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12970.12044332802466443No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGTTGGTA12880.11960756090652874No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCA12880.11960756090652874No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAA12860.11942183488027636No Hit
CTACTTAACTTGGTACCAACAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATC12540.11645021846023837No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12460.11570731435522888No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12150.11282856094831709No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCATATTTTATACAC12080.11217851985643379No Hit
CCTCCAACAATAACAACTACTTAACTTGGTACCAACAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGG11530.1070710541344935No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11500.10679246509511493No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11410.10595669797697925No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11260.10456375278008645No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT11240.10437802675383409No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10970.10187072539942703No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10830.10057064321566042No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10810.10038491718940803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC352.557942E-548.3102073
GGTATCA11650.043.251111
GTCGTAT702.2502718E-839.305239
GTCACCG5450.036.1601570
TCGTATG2350.035.20025640
GTATCAA27300.035.0558661
CGTATGC2200.034.54047841
CCCACGA3000.033.81714219
ACGAGAC3050.033.28130722
ATGCCGT2550.032.68696644
AATGTCG851.3029967E-732.1926436
GCCGTCT2600.032.17189846
GTAATAC5850.031.2158241
CCGTCTT2800.031.17385347
TGGTATC3600.030.9990462
TATCAAC31400.030.5862052
TGTCGTA902.0855623E-730.51163338
ATCAACG31600.030.3926243
TCAACGC31850.030.1540624
AGCCGTG6000.029.8718110
Produced by FastQC (version 0.11.5)