FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360661
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACAGCCGTGTCTTCGGCTGTGAGGCTGTCCATTTGCAGATAGA73680.5415015202170121No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45650.3354987024688736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44410.32638548470192064No Hit
GTCTACTACTGTGCGAGGGATTTGCAGTTCAAGTTTGGCCATGACTCCTGGGGCCAGGGCACCCTGGTCA44160.3245481424101962No Hit
GTACCAACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAA39750.29213742438417795No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33110.24333761311597818No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC31980.23503282595738395No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28720.2110738824732979No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27620.2029895763897106No Hit
GTCTGGGACAGACTTCACTCTCACCGTCACCGGACTGCAGCCCGAGGATT27000.1984329675062341No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26600.19549321983947507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26420.1941703333894335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26360.19372937123941966No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGACACGAACTGTGGCTGCACCA25880.19020167403930882No Hit
GTGTTGGTACCAGGCTAAATAGTCGGTGCCAAGACTCTGACTGGCCCTGC24890.18292579856408026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24840.18255833010573538No Hit
GTCCCAGACCCACTGCCAGTGAATCTGTTTGGGATGTCAGTGGCCCTATT24230.17807521491392786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24150.17748726538057608No Hit
ACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATT23860.17535594832217577No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23410.17204873219707187No Hit
CTCCTGCACGGCCTCTGGATTCAGGTTCAGTAGTTACAACATGAATTGGGTCCGCCAGGCTCCAGGAAAGGGC23350.17160777004705802No Hit
GGCCAAAAGTGTACGGCGGTCGACCAAATTGTTGACAGTAATAGGTCGCAAAATCCTCGGGCTGCAGTCCGGTGA22880.16815356653861616No Hit
CTATTACTGTCAACAATTTGGTCGACCGCCGTACACTTTTGGCCAGGGGA22400.16462586933850532No Hit
ATATAGGAGGAGTCTGGGAGCCTGGCCAGGTCTGTGTTGGTACCAGGCTAAATAGTCGGTGCCAAGACTCTGA22130.162641539663443No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21240.15610060110490417No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21170.15558614526322134No Hit
CCTGACACCCTGTCTGTGTCTCCAGGCGAAACTGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTGGCACCGA20750.15249941021312435No Hit
ACCTATTACTGTCAACAATTTGGTCGACCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGACACGAA20160.1481632824046548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20150.1480897887129858No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20110.14779581394630992No Hit
CCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATTCACTGGCAGTGGGTCTGGGACAGACTT19990.14691388964628221No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGATCTGGGGCTCCGCTGTG19990.14691388964628221No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19810.14559100319624066No Hit
GTGCAGCCACAGTTCGTGTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG19780.14537052212123372No Hit
GACTTCACTCTCACCGTCACCGGACTGCAGCCCGAGGATTTTGCGACCTATTACTGTCAACAATTTGGTCGACCG19700.1447825725878819No Hit
GTAATAGGTCGCAAAATCCTCGGGCTGCAGTCCGGTGACGGTGAGAGTGA19420.1427247492211506No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC19010.1397115078627226No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18800.1381681403376741No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18680.1372862160376464No Hit
GTCTTGGCACCGACTATTTAGCCTGGTACCAACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCAT18080.13287659453750789No Hit
CCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATTCACTGGCAGTGGGTCTGGGACAGACTTCA17750.1304513027124317No Hit
CTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATTCAC17330.1273645676623347No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG17270.12692360551232085No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17220.12655613705397598No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17220.12655613705397598No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17210.126482643362307No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16970.12471879476225159No Hit
CAGTAATAGGTCGCAAAATCCTCGGGCTGCAGTCCGGTGACGGTGAGAGT16900.12420433892056876No Hit
GGTCAAGCCTGCGGGGTCCCTGAGACTCTCCTGCACGGCCTCTGGATTCA16730.12295494616219617No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATAT16690.12266097139552028No Hit
GCACTGAGCACCGAGGATTCACCATGGATCTGGGGCTCCGCTGTGTTTTCCTTGTTGCTATTTTAGAAGGTGTC16650.12236699662884437No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16620.12214651555383745No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16580.12185254078716154No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16350.12016218587877509No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16150.1186923120453956No Hit
CTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCA16100.1183248435870507No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAATCTGTTTGGGATGTCA16010.11766340036202994No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15860.11656099498699528No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATATCGTCGGAGAAGTTGTGTTGACGCAGTCTC15850.11648750129532631No Hit
GTCTCCTGACACCCTGTCTGTGTCTCCAGGCGAAACTGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTGGCAC15750.11575256437863655No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15680.11523810853695372No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15410.11325377886189139No Hit
GACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCC15320.1125923356368706No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15280.11229836087019471No Hit
CTCCAGGGACAACGCCGAGAATACAGTCTATCTGCAAATGGACAGCCTCACAGCCGAAGACACGGCTGTCTACTA15270.11222486717852574No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15230.11193089241184984No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15210.11178390502851188No Hit
GGTCTGTGTTGGTACCAGGCTAAATAGTCGGTGCCAAGACTCTGACTGGCCCTGCAGAAGAGGGTGGCAGTTTCG15050.11060800596180828No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14950.10987306904511852No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14710.1081092204450631No Hit
GTCTGTGTCTCCAGGCGAAACTGCCACCCTCTTCTGCAGGGCCAGTCAGA14500.10656585292001461No Hit
GTGTACGGCGGTCGACCAAATTGTTGACAGTAATAGGTCGCAAAATCCTC14210.10443453586161432No Hit
CCTTGGGGCTGGTCGGGGATGCGGGAGAGACAGTGACCAGGGTGCCCTGG13950.10252369987822096No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC13820.10156828188652427No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13710.10075985127816554No Hit
CTATTTAGCCTGGTACCAACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCA13650.10031888912815168No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13650.10031888912815168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAA200.007107719352.20721445
TCGAATA200.007171855352.0887544
GTCGTAT352.3229795E-549.1050639
CGTATGC2100.047.6842241
TCGTATG2000.046.50351340
ATCTCGT1850.044.3916337
GGTATCA13350.041.7250371
ATGCCGT2300.040.7651144
CCGTCTT2350.040.15287847
ACGAGAC2750.039.2879422
GTATGCC2600.038.59545542
CCCACGA2800.038.5750219
CGTCTTC2100.038.3623948
TACGTAT700.001589765338.12376870
TAAGACA2850.038.1083931
ATCTCCG2750.038.04200710
CACGAGA2850.037.9038421
TATACAC2900.037.2421073
TTATACA2850.036.7112462
CATCTCG2250.036.44274536
Produced by FastQC (version 0.11.5)