FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360661
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACAGCCGTGTCTTCGGCTGTGAGGCTGTCCATTTGCAGATAGA67460.49578844399890937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45730.33608665200222543No Hit
GTCTACTACTGTGCGAGGGATTTGCAGTTCAAGTTTGGCCATGACTCCTGGGGCCAGGGCACCCTGGTCA44420.32645897839358956No Hit
GTACCAACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAA37990.2792025346504383No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37180.2732495456252512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36240.26634113860836756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33010.24260267619928846No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC31560.23194609090728696No Hit
GTCTGGGACAGACTTCACTCTCACCGTCACCGGACTGCAGCCCGAGGATT28310.20806064111486988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28180.20710522312317325No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26880.1975510432062064No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26760.19666911890617872No Hit
GTGTTGGTACCAGGCTAAATAGTCGGTGCCAAGACTCTGACTGGCCCTGC26500.19475828292278533No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25430.18689445791420495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24760.18197038057238357No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGACACGAACTGTGGCTGCACCA24570.180574000430673No Hit
ACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATT23260.17094632682203723No Hit
CTCCTGCACGGCCTCTGGATTCAGGTTCAGTAGTTACAACATGAATTGGGTCCGCCAGGCTCCAGGAAAGGGC22790.16749212331359536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22790.16749212331359536No Hit
GTCCCAGACCCACTGCCAGTGAATCTGTTTGGGATGTCAGTGGCCCTATT22240.16344997027180172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21230.15602710741323517No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20970.15411627142984183No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20880.15345482820482104No Hit
GGCCAAAAGTGTACGGCGGTCGACCAAATTGTTGACAGTAATAGGTCGCAAAATCCTCGGGCTGCAGTCCGGTGA20810.1529403723631382No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20510.15073556161306895No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20360.1496331562380343No Hit
CTATTACTGTCAACAATTTGGTCGACCGCCGTACACTTTTGGCCAGGGGA20260.14889821932134456No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19740.14507654735455783No Hit
ACCTATTACTGTCAACAATTTGGTCGACCGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGACACGAA19650.14441510412953704No Hit
CCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATTCACTGGCAGTGGGTCTGGGACAGACTT19610.14412112936286114No Hit
GACTTCACTCTCACCGTCACCGGACTGCAGCCCGAGGATTTTGCGACCTATTACTGTCAACAATTTGGTCGACCG19460.1430187239878265No Hit
ATATAGGAGGAGTCTGGGAGCCTGGCCAGGTCTGTGTTGGTACCAGGCTAAATAGTCGGTGCCAAGACTCTGA19460.1430187239878265No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT19420.1427247492211506No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGATCTGGGGCTCCGCTGTG19190.14103439431276416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.13838862141268105No Hit
GTAATAGGTCGCAAAATCCTCGGGCTGCAGTCCGGTGACGGTGAGAGTGA18760.13787416557099821No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.13625730435428077No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18400.1352283926709151No Hit
GTGCAGCCACAGTTCGTGTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18140.13331755668752174No Hit
GTCTTGGCACCGACTATTTAGCCTGGTACCAACACAGACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCAT17950.13192117654581118No Hit
CCTGACACCCTGTCTGTGTCTCCAGGCGAAACTGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTGGCACCGA17750.1304513027124317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17410.1279525171956865No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17400.12787902350401753No Hit
CTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATTCAC17220.12655613705397598No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17050.1253067442956034No Hit
CCTATATGGTTCATCAAATAGGGCCACTGACATCCCAAACAGATTCACTGGCAGTGGGTCTGGGACAGACTTCA16910.12427783261223774No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16430.1207501354121269No Hit
GGTCAAGCCTGCGGGGTCCCTGAGACTCTCCTGCACGGCCTCTGGATTCA16280.11964773003709227No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAATCTGTTTGGGATGTCA15930.11707545082867811No Hit
GTCTGTGTCTCCAGGCGAAACTGCCACCCTCTTCTGCAGGGCCAGTCAGA15700.11538509592029168No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATAT15690.11531160222862272No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15640.11494413377027782No Hit
GCACTGAGCACCGAGGATTCACCATGGATCTGGGGCTCCGCTGTGTTTTCCTTGTTGCTATTTTAGAAGGTGTC15490.1138417283952432No Hit
CTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCCACTGACATCCCA15480.11376823470357422No Hit
GTCTCCTGACACCCTGTCTGTGTCTCCAGGCGAAACTGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTGGCAC15150.11134294287849801No Hit
CAGTAATAGGTCGCAAAATCCTCGGGCTGCAGTCCGGTGACGGTGAGAGT15030.11046101857847032No Hit
GGTCTGTGTTGGTACCAGGCTAAATAGTCGGTGCCAAGACTCTGACTGGCCCTGCAGAAGAGGGTGGCAGTTTCG15030.11046101857847032No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA14930.10972608166178055No Hit
GACCTGGCCAGGCTCCCAGACTCCTCCTATATGGTTCATCAAATAGGGCC14910.10957909427844262No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14840.10906463843675979No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14840.10906463843675979No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTCCTCTGGCTCCCAGATATCGTCGGAGAAGTTGTGTTGACGCAGTCTC14790.1086971699784149No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14740.10832970152007003No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14740.10832970152007003No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14630.10752127091171129No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14520.10671284030335257No Hit
CCTTGGGGCTGGTCGGGGATGCGGGAGAGACAGTGACCAGGGTGCCCTGG14450.10619838446166974No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14320.10524296646997305No Hit
CTCCAGGGACAACGCCGAGAATACAGTCTATCTGCAAATGGACAGCCTCACAGCCGAAGACACGGCTGTCTACTA14240.10465501693662126No Hit
GTGTACGGCGGTCGACCAAATTGTTGACAGTAATAGGTCGCAAAATCCTC14140.10392008001993149No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14030.10311164941157276No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13950.10252369987822096No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC13680.10053937020315862No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13640.10024539543648271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)