FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794999
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33080.41610115232849343No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28070.3530822051348492No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26400.33207588940363447No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23690.2979877962110644No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20410.25672988267909774No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20310.2554720194616597No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG20150.2534594383137589No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20020.25182421613108946No Hit
GACTATTACTGCAGCTCATATACAAGCACGAACACGGCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGT19730.24817641280051925No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19610.2466669769395936No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19400.2440254641829738No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGC18820.23672985752183337No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17800.22389965270396567No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTA17750.22327072109524665No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17180.21610090075585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17110.2152203965036434No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTACTGCAGCTCATAT17080.214843037538412No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16970.2134593879992302No Hit
CTATTACTGCAGCTCATATACAAGCACGAACACGGCGGTCTTCGGAACTG16400.20628956765983356No Hit
GTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15240.19169835433755264No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC14420.18138387595456096No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAT14080.17710714101527172No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13980.17584927779783371No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13960.1755977051543461No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGCCGTGTTCGTGCTTGTATATGAGCTGCAGTAATAGT13860.17433984193690807No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA13850.17421405561516431No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13470.16943417538889985No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13320.16754738056274285No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13250.16666687631053623No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13200.16603794470181724No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGG13000.16352221826694122No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12920.1625159276929908No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12870.16188699608427182No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12820.1612580644755528No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12010.1510693724143049No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11530.14503162897060246No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11460.1441511247183959No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGCCGTGTTCGTGC11330.14251590253572646No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11190.14075489403131325No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11050.13899388552690004No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11020.13861652656166865No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGG10890.13698130437899922No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10740.13509450955284222No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10610.1334592873701728No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG9800.12327059530892492No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9710.1221385184132307No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9660.12150958680451171No Hit
CTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA9630.12113222783928031No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9620.1210064415175365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9320.11723285186522248No Hit
GTTCCGAAGACCGCCGTGTTCGTGCTTGTATATGAGCTGCAGTAATAGTC9320.11723285186522248No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTTTCTAATCGC9290.11685549289999107No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC9280.11672970657824727No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9280.11672970657824727No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9190.11559762968255308No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9160.11522027071732166No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9120.11471712543034646No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9100.11446555278685885No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9070.11408819382162745No Hit
GTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGA9060.11396240749988365No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTACTGCAGCTCATATACAAGCACGAACACGGCGGT8960.11270454428244565No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGCCGTGTTCGTGCTTGTATATGAGCTG8940.11245297163895804No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8840.11119510842152003No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8720.10968567256059443No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8490.10679258716048701No Hit
GTACTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA8420.10591208290828039No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8400.10566051026479278No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATT8390.10553472394304898No Hit
GTAATCGGCCCTCAGGGGTTTCTAATCGCCTCTCTGGCTCCAAGTCTGGC8370.10528315129956138No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8290.10427686072561097No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8260.10389950176037958No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8250.10377371543863578No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC8210.10327057015166058No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG8170.10276742486468536No Hit
ATATTATTGTGCGGCAGAACTGAATTACTATGATCGTAGTGGTTACTTCCACCGGAGTGAGAGTGCTTTTGAGAT8160.10264163854294156No Hit
CCCCTGAGGGCCGATTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAGGAGA8160.10264163854294156No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG8150.10251585222119776No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8120.10213849325596636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG200.00673172352.92759757
TCGAGAA200.00722961451.98119443
GTGGTAT1950.050.765331
TCTCGTA850.048.6068638
CGTATGC800.047.5189441
GCTAATC800.047.11684833
ATCTCCG950.046.70559710
GGTATCA8800.046.1602441
TATACAC1150.044.5243153
TCGTATG950.043.61487640
ATGCCGT803.274181E-1143.3758344
TGGTATC2300.043.0401732
CGAGCCC1050.042.25744615
TCTCCGA1050.042.25744611
GTATCAA25400.040.854861
TGGGCGC350.001181924240.49430559
CTTATAC1350.040.456661
ACGAGAC1100.040.34680622
CCCACGA1100.040.33918819
TCCGAGC1100.040.3366513
Produced by FastQC (version 0.11.5)