FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794999
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28200.3547174273175186No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27780.349434401804279No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25690.3231450605598246No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21560.27119530967963484No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG21180.2664154294533704No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20900.26289341244454395No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20440.25710724164432913No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19220.2417613103915854No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19060.23974872924368457No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18720.23547199430439536No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18300.23018896879115572No Hit
GACTATTACTGCAGCTCATATACAAGCACGAACACGGCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGT17310.2177361229385194No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17030.214214105929693No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGC16930.21295624271225497No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16130.20289333697275092No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTA15560.19572351663335427No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTACTGCAGCTCATAT15270.19207571330278403No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14670.18452853399815597No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14650.18427696135466837No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14430.18150966227630475No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTC14190.17849079055445352No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAT14100.1773587136587593No Hit
GTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13630.17144675653680067No Hit
CTATTACTGCAGCTCATATACAAGCACGAACACGGCGGTCTTCGGAACTG13130.16515744044961064No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13060.16427693619740402No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12830.1613838507972966No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12810.161132278153809No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGCCGTGTTCGTGCTTGTATATGAGCTGCAGTAATAGT12700.15974862861462719No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12640.15899391068416438No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12050.15157251770128014No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA11940.1501888681620983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11660.14666685115327188No Hit
GCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGG11640.14641527850978428No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11500.1446542700053711No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11020.13861652656166865No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10770.1354718685180736No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10680.13433979162237938No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG10560.1328303557614538No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10480.1318240651875034No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10310.12968569771785876No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGG10220.12855362082216457No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10150.12767311656995795No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9970.12540896277856953No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9910.12465424484810672No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGCCGTGTTCGTGC9610.1208806551957927No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9430.11861650140440429No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9380.11798756979568528No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9300.11698127922173487No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9250.11635234761301586No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8960.11270454428244565No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGCCGTGTTCGTGCTTGTATATGAGCTG8920.11220139899547044No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8840.11119510842152003No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8830.11106932209977623No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG8800.11069196313454482No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG8800.11069196313454482No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8770.11031460416931341No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8760.1101888178475696No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8590.108050450377925No Hit
CTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA8510.10704415980397458No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8510.10704415980397458No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8410.10578629658653659No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8400.10566051026479278No Hit
GTATATGAGCTGCAGTAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGA8350.10503157865607378No Hit
GTTCCGAAGACCGCCGTGTTCGTGCTTGTATATGAGCTGCAGTAATAGTC8350.10503157865607378No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAATCGGCCCTCAGGGGTTTCTAATCGC8280.10415107440386717No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTACTGCAGCTCATATACAAGCACGAACACGGCGGT8280.10415107440386717No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC8280.10415107440386717No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8170.10276742486468536No Hit
GTACTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA8130.10226427957771016No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8070.10150956164724735No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7990.10050327107329696No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC7970.10025169842980935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)