FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856855
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCCTACACTTTTGGCCAGGGGACGAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT81780.44042211158114125No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73430.3954536030007728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60050.32339628026959566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58930.31736457612468394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58580.315479668579399No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT54280.29232223302304167No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA53780.2896295079583489No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46770.251877502551357No Hit
CATCTATGCTGCTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA46260.24913092298537043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44650.24046034827705987No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT44470.2394909672537705No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG43990.23690595119166546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43500.23426708062826662No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43030.23173591906745544No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41910.22570421492254375No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT40660.21897240226081194No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39930.2150410236663606No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38220.2058319039451115No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37980.20453939591405898No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACATCAACGTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT37680.20292376087524336No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA36670.19748445624456407No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34340.184936357443096No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT34320.18482864844050828No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAT34230.18434395792886357No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33980.18299759539651722No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33440.18008945232664908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32870.1770197457528994No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCCTACACTTTTGGCCA32590.17551181971667149No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32220.17351920316879885No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32010.1723882586416279No Hit
GTATTAACATCAACGTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCTTCCA31650.17044949659504915No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACATCAACGTAGCCTGGTA31320.16867229805235195No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31120.16759520802647487No Hit
GAGTATTAACATCAACGTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCTTC30670.16517175546825144No Hit
CCCTACACTTTTGGCCAGGGGACGAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG29630.15957088733369057No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC29270.1576321252871118No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTCGTCC28690.15450856421206827No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGC28440.15316220167972192No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA27930.15041562211373533No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA27780.14960780459432751No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG26790.1442762089662359No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26370.14201431991189403No Hit
GTCTCTGGTGGCTCCATCAATGATTACTACTGGAGCTGGATCCGGCAGTC26140.14077566638213537No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25740.1386214863303812No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTCGTCCCCTGGCCAAAAGTGTAGGGAGGCCAGTTATTATACTG25070.13501323474369298No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25050.13490552574110526No Hit
GTGTAGGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATC25040.1348516712398114No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24830.13372072671264046No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT24460.13172811016476785No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24420.13151269215959244No Hit
CTTTTGGCCAGGGGACGAAGGTGGAGATCAAACGAACTGTGGCTGCACCA24370.13124341965312314No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24220.13043560213371533No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC23520.12666578704314552No Hit
GGATACCAGTGGCCCTGGTGGAAGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGG23090.1243500434875098No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23070.12424233448492208No Hit
GGCCAAAAGTGTAGGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG22520.12128033691376008No Hit
GCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCTTCCACCAGGGCCACTGGTATC22230.11971855637623832No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22210.11961084737365062No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21960.11826448484130425No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21880.11783364883095342No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21480.11567946877919924No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21320.11481779675849757No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20840.11223278069639256No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20780.11190965368862943No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20350.10959391013299369No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20260.10910921962134901No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20200.10878609261358588No Hit
GTATTACTGTGCGAGGGGTGTTTTGATTACCTTTGGGGGAGATATCGCAACCAAGGGATCTGATGTTTTTGATAT20150.10851682010711661No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19970.10754743908382722No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19810.10668576706312555No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19790.10657805806053783No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTGGACTTTTCTA19620.10566253153854231No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19570.10539325903207306No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC19470.1048547140191345No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18880.1016772984427971No Hit
GGGGACGAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA18780.10113875342985855No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACATCAACGTAGC18740.10092333542468312No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAACATCAACGTAGCCTGGTACCAGCAAAAACCTGGCCAGG18650.10043864491303844No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC18640.10038479041174458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACT209.217167E-567.9054626
GGTATCA20050.043.281021
TACTGAT13400.038.58852870
GTATCAA48350.034.3535421
TATCAAC52150.031.3826222
TCAACGC55250.029.7372364
CAACGCA56000.029.338975
AACGCAG56100.029.286676
CGGTACC953.01432E-729.25746545
AATGGTA7250.029.0919770
GAGTTTA6500.028.1612741
ACGCAGA58800.027.9418757
CGCAGAG59400.027.7144248
GACAGGT4100.027.2836191
TCTACTG11550.027.05622368
AGAGTAC60750.026.98703211
GATTCAC4300.026.8029371
TAACGGC1156.062692E-826.80100636
GCAGAGT61300.026.8001549
TTAACGG1156.1188985E-826.77549635
Produced by FastQC (version 0.11.5)