FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995855
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCGTCGTCACTCAGCTTGTTCATTTGCAGATAGA49660.49866697460975745No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29860.299842848607478No Hit
GTGTATTACTGTGCGAAAGATCATGACTGGAACTTTTCTGTCTGGGGCCA26600.26710715917477945No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTC25760.2586721962534706No Hit
GTATTACTGTCAGCAGTTTGCTAACTCACCCATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCCACCGAA25150.2525468065129964No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22700.22794483132584564No Hit
GTGTATTACTGTCAGCAGTTTGCTAACTCACCCATGTGCAGTTTTGGCCA20620.2070582564730809No Hit
CAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18870.18948541705368752No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18360.18436418956575001No Hit
CAGTAATACACGGCCGCGTCGTCGTCACTCAGCTTGTTCATTTGCAGATA17770.17843963227578313No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17430.1750254806171581No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA17090.17161132895853312No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16510.16578718789381988No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16270.16337719848773163No Hit
CTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTACACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG16040.16106762530689708No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15980.16046512795537504No Hit
GTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15910.1597622143785993No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15570.1563480627199743No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13910.139678969327864No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT13250.13305149846112135No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12870.12923568190148163No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12790.12843235209945222No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12450.12501820044082723No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12160.12210612990847061No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12130.12180488123270958No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACAGAAAAGTTCCAGTCATGATCTTTCGCACAGTAATA12130.12180488123270958No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCAGCCACTTAGCCTGGTACC12080.12130280010644119No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12080.12130280010644119No Hit
CCATTAGCACCTACACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGCGATGGGTCTCGTCTGTAAATA11890.11939489182662136No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11890.11939489182662136No Hit
GTATTACTGTGCGAAAGATCATGACTGGAACTTTTCTGTCTGGGGCCAGG11860.11909364315086032No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC11720.11768781599730885No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT11590.11638240506901104No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC11580.11628198884375737No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11460.11507699414071326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11360.11407283188817649No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC11240.11286783718513238No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT11230.11276742095987871No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT11150.11196409115784929No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10960.11005618287802943No Hit
GAGTTTGGGCTGAGCTGGCTTTTCCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTTTTGGAGT10850.10895160440023899No Hit
CACCTACACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGCGAT10770.10814827459820957No Hit
GGGTTCCCTGGCCCCAGACAGAAAAGTTCCAGTCATGATCTTTCGCACAGTAATACACGGCCGCGTCGTCGTCA10740.10784702592244855No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTCCTTGT10710.10754577724668751No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCG10680.10724452857092648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10600.10644119876889709No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10510.10553745274161398No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA10490.10533662029110664No Hit
GCCCTGGGATTTTCAGGTGTTTTCACTTGGTGATCAGGACTGAACAGAGA10430.10473412293958459No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC10330.10372996068704782No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG10210.1025249659840037No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTACACCATGACCTGGGTCCG10190.10232413353349634No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG10180.10222371730824267No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10140.10182205240722796No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10030.10071747392943753No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9970.10011497657791545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)